The working mechanism of an immune complex vaccine that protects chickens against infectious bursal disease
| Autor: | Eid E. Haddad, E. M. Janse, S. H. M. Jeurissen, Avakian Alan P, C. Whitfill, P. R. Lehrbach |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
animal structures
Immunology Spleen Antigen-Antibody Complex Biology Virus Replication In ovo Infectious bursal disease virus Virus Infectious bursal disease Immunoenzyme Techniques Bursa of Fabricius Immune system medicine Life Science Animals Immunology and Allergy Instituut voor Dierhouderij en Diergezondheid Poultry Diseases B-Lymphocytes ID-Lelystad Viral Vaccine Germinal center Viral Vaccines Dendritic Cells Birnaviridae Infections medicine.disease Virology ID Lelystad medicine.anatomical_structure ID-Lelystad Instituut voor Dierhouderij en Diergezondheid ID Lelystad Institute for Animal Science and Health Chickens Institute for Animal Science and Health Research Article |
| Zdroj: | Immunology, 95(3), 494-500 Immunology 95 (1998) 3 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.1998.00617.x |
| Popis: | The role of immune complexes (Icx) in B-cell memory formation and affinity maturation allow for their potential use as vaccines. Recently, a new immune complex vaccine has been developed that is currently under field trials conducted in commercial poultry. This immune complex vaccine is developed by mixing live intermediate plus infectious bursal disease virus (IBDV) with hyperimmune IBDV chicken serum (IBDV-Icx vaccine). Here we have investigated the infectivity of this vaccine as well as the native IBDV (uncomplexed) vaccine in terms of differences in target organs, in target cells and speed of virus replication. At various days after inoculation on day 18 of incubation (in ovo) with either one dose of virus alone or the IBDV-Icx vaccine, the replication of IBDV and the frequency of B cells and other leucocyte populations were examined in the bursa of Fabricius, spleen, and thymus using immunocytochemistry. With both vaccines, IBDV was detected associated with B cells, macrophages and follicular dendritic cells (FDC) in bursa and spleen, although complexing IBDV with specific antibodies caused a delay in virus detection of about 5 days. Most remarkable was the low level of depletion of bursal and splenic B cells in IBDV-Icx vaccinated chickens. Furthermore, in ovo inoculation with the IBDV-Icx vaccine induced more germinal centres in the spleen and larger amounts of IBDV were localized on both splenic and bursal FDC. From these results we hypothesize that the working mechanism of the IBDV-Icx vaccine is related to its specific cellular interaction with FDC in spleen and bursa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|