A human preadipocyte cell strain with multipotent differentiation capability as an in vitro model for adipogenesis
| Autor: | Angélica Montoya-Contreras, Luis A. Salazar-Olivo, Kristina Mäemets-Allas, Viljar Jaks, Claudia G. Cárdenas-León |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Pluripotent Stem Cells 0301 basic medicine Homeobox protein NANOG Stromal cell Carcinogenesis Karyotype Population Adipose tissue Biology Models Biological Cell Line Kruppel-Like Factor 4 Mice 03 medical and health sciences 0302 clinical medicine Adipocytes Animals Humans Doubling time Cell Lineage education Cellular Senescence Cell Proliferation education.field_of_study Adipogenesis Multipotent Stem Cells Infant Cell Biology General Medicine beta-Galactosidase Diploidy Cell biology 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Stem cell Chondrogenesis Biomarkers Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 56:399-411 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-020-00468-z |
| Popis: | Murine 3T3 cell lines constitute a standard model system for in vitro study of mammalian adipogenesis although they do not faithfully reflect the biology of the human adipose cells. Several human adipose cell lines and strains have been used to recapitulate human adipogenesis in vitro, but to date there is no generally accepted in vitro model for human adipogenesis. We obtained a clonal strain of human subcutaneous adipose stromal cells, IPI-SA3-C4, and characterized its utility as an in vitro model for human subcutaneous adipogenesis. IPI-SA3-C4 cells showed a high proliferative potential for at least 30 serial passages, reached 70 cumulative population doublings and exhibited a population doubling time of 47 h and colony forming efficiency of 12% at the 57th cumulative population doublings. IPI-SA3-C4 cells remained diploid (46XY) even at the 56th cumulative population doublings and expressed the pluripotency markers POU5F1, NANOG, KLF4, and MYC even at 50th cumulative population doublings. Under specific culture conditions, IPI-SA3-C4 cells displayed cellular hallmarks and molecular markers of adipogenic, osteogenic, and chondrogenic lineages and showed adipogenic capacity even at the 66th cumulative population doublings. These characteristics show IPI-SA3-C4 cells as a promising potential model for human subcutaneous adipogenesis in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink