In vitro cytotoxicity induced by Clostridium perfringens isolate carrying a chromosomal cpe gene is exclusively dependent on sporulation and enterotoxin production
| Autor: | Mayo Yasugi, Mahfuzur R. Sarker, Kaori Kondo, Yuki Sugahara, Prabhat K. Talukdar, Shigeki Yamamoto, Hidenobu Hoshi, Yoichi Kamata, Masami Miyake |
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| Rok vydání: | 2014 |
| Předmět: |
Programmed cell death
Somatic cell Clostridium perfringens viruses Mutant Enterotoxin Biology medicine.disease_cause Microbiology Foodborne Diseases Enterotoxins stomatognathic system Bacterial Proteins medicine Cytotoxic T cell Humans Cytotoxicity Spores Bacterial Virulence urogenital system Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition Chromosomes Bacterial In vitro Infectious Diseases Caco-2 Cells Gas Gangrene |
| Zdroj: | Microbial pathogenesis. 85 |
| ISSN: | 1096-1208 |
| Popis: | Clostridium perfringens type A is a common source of food poisoning (FP) and non-food-borne (NFB) gastrointestinal diseases in humans. In the intestinal tract, the vegetative cells sporulate and produce a major pathogenic factor, C. perfringens enterotoxin (CPE). Most type A FP isolates carry a chromosomal cpe gene, whereas NFB type A isolates typically carry a plasmid-encoded cpe. In vitro, the purified CPE protein binds to a receptor and forms pores, exerting a cytotoxic activity in epithelial cells. However, it remains unclear if CPE is indispensable for C. perfringens cytotoxicity. In this study, we examined the cytotoxicity of cpe-harboring C. perfringens isolates co-cultured with human intestinal epithelial Caco-2 cells. The FP strains showed severe cytotoxicity during sporulation and CPE production, but not during vegetative cell growth. While Caco-2 cells were intact during co-culturing with cpe-null mutant derivative of strain SM101 (a FP strain carrying a chromosomal cpe gene), the wild-type level cytotoxicity was observed with cpe-complemented strain. In contrast, both wild-type and cpe-null mutant derivative of the NFB strain F4969 induced Caco-2 cell death during both vegetative and sporulation growth. Collectively, the Caco-2 cell cytotoxicity caused by C. perfringens strain SM101 is considered to be exclusively dependent on CPE production, whereas some additional toxins should be involved in F4969-mediated in vitro cytotoxicity. |
| Databáze: | OpenAIRE |
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