An expanded surface-enhanced Raman scattering tags library by combinatorial encapsulation of reporter molecules in metal nanoshells
| Autor: | Luis M. Liz-Marzán, Jorge Pérez-Juste, Sergio Rodal-Cedeira, Sara Bals, Alba Vázquez-Arias, Isabel Pastoriza-Santos, Sara Nunez-Sanchez, Lakshminarayana Polavarapu, Andrea Laporta, Alexander Skorikov, Gustavo Bodelón |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
In situ
Materials science General Physics and Astronomy Metal Nanoparticles plasmonic nanocapsules Nanotechnology 2210 Química Física 02 engineering and technology 010402 general chemistry Spectrum Analysis Raman 01 natural sciences Nanocapsules Article symbols.namesake cell imaging Molecule General Materials Science Sulfhydryl Compounds SERS library Nanoshells Physics General Engineering technology industry and agriculture 021001 nanoscience & nanotechnology Nanoshell 0104 chemical sciences Chemistry Electron tomography SERS tags Colloidal gold symbols Gold 0210 nano-technology Raman spectroscopy Engineering sciences. Technology Raman scattering combinatorial encapsulation |
| Zdroj: | ACS nano ACS Nano |
| ISSN: | 1936-0851 |
| Popis: | Raman-encoded gold nanoparticles (NPs) have been widely employed as photostable multifunctional probes for sensing, bioimaging, multiplex diagnostics, and surfaceenhanced Raman scattering (SERS)-guided tumor therapy. We report a strategy toward obtaining a particularly large library of Au nanocapsules encoded with Raman codes defined by the combination of different thiol-free Raman reporters, encapsulated at defined molar ratios. The fabrication of SERS tags with tailored size and predefined codes is based on the in situ incorporation of Raman reporter molecules inside Au nanocapsules during their formation "via" galvanic replacement coupled to seeded growth on Ag NPs. The hole-free closedshell structure of the nanocapsules is confirmed by electron tomography. The unusually wide encoding possibilities of the obtained SERS tags are investigated by means of either wavenumber-based encoding or Raman frequency combined with signal intensity, leading to an outstanding performance as exemplified by 26 and 54 different codes, respectively. We additionally demonstrate that encoded nanocapsules can be readily bioconjugated with antibodies for applications such as SERS-based targeted cell imaging and phenotyping. Ministerio de Ciencia, Innovación y Universidades | Ref. MDM-2017-0720 Ministerio de Economía, Industria y Competitividad | Ref. MAT2016-77809-R Agencia Estatal de Investigación | Ref. PID2019-108954RB-100 Xunta de Galicia | Ref. ED431G 2019/07 Fundación Ramón Areces Research Foundation Flanders |
| Databáze: | OpenAIRE |
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