An Animal Model of Necrotizing Enterocolitis (NEC) in Preterm Rabbits
| Autor: | Jeffrey M. Burford, Melvin S. Dassinger, Rhea J. Birusingh, Samuel D. Smith, Andrew P. Bozeman |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Day of life Gastroenterology Pathology and Forensic Medicine Ranitidine Animal model Enterocolitis Necrotizing Internal medicine medicine Animals Large intestine Enterocolitis business.industry Incidence (epidemiology) General Medicine medicine.disease digestive system diseases Nonsurgical treatment Surgery Disease Models Animal medicine.anatomical_structure Animals Newborn Pediatrics Perinatology and Child Health Necrotizing enterocolitis Premature Birth Rabbits medicine.symptom business medicine.drug |
| Zdroj: | Fetal and Pediatric Pathology. 32:113-122 |
| ISSN: | 1551-3823 1551-3815 |
| Popis: | Creation of an animal model of necrotizing enterocolitis (NEC) allowing adjustment of severity and potential recoverability is needed to study effectiveness of prevention and treatment strategies. This study describes a novel model in preterm rabbits capable of adjusting severity of NEC-like histologic changes. Rabbit pups (n = 151) were delivered by cesarean section 2 days preterm. In the treatment groups, tissue adhesive was applied to anal openings to simulate the poor intesti- nal function and dysmotility of preterm neonates. Pups were placed into five groups: 3INT (3 day intermittent block), 4INT (4 day intermittent block), 3COM (3 day complete block), 4COM (4 day complete block), based on differences in type of anal blockage and day of life sacrificed. The fifth group, 4CON, was comprised of a control arm (n = 28) without anal block, with sacrifice of sub- jects on day 4. All pups were gavage fed with formula contaminated with Enterobacter cloacae, ranitidine, and indomethacin. Following sacrifice, the intestines were harvested for pathologic evidence of NEC. A blinded pathologist graded histologic changes consistent with NEC using a grading scale 0-4 with 4 being most severe. Fifty-seven pups (57/123) (46%) in the research arm survived to sacrifice, compared to 26/28 (93%) in the control arm of the investigation, p < 0.0001. The incidence and severity of NEC-like damage increased with the duration and completeness of the anal blockage. 44/57 (77%) of survivors revealed various degrees of NEC-like damage to large and small bowel, and 3/26 (12%) exhibited early NEC-like mucosal injury in the research and control arms, respectively. This animal model produces NEC-like pathologic changes in both small and large intestine in preterm rabbits. Because incidence and severity of damage increases with duration and completeness of intestinal dysmotility, this allows future effectiveness studies for nonsurgical treatment and prevention of NEC. |
| Databáze: | OpenAIRE |
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