Identification of an alternatively spliced isoform of the fyn tyrosine kinase
| Autor: | Jeffrey F. Goldsmith, T. Prescott Atkinson, Craig G Hall |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Gene isoform
Biophysics Biology Proto-Oncogene Proteins c-fyn Biochemistry Jurkat cells Exon Jurkat Cells FYN Proto-Oncogene Proteins Animals Humans RNA Messenger Molecular Biology DNA Primers COS cells Base Sequence Reverse Transcriptase Polymerase Chain Reaction Alternative splicing Cell Biology U937 Cells Molecular biology Isoenzymes Alternative Splicing RNA splicing COS Cells Tyrosine kinase |
| Zdroj: | Biochemical and biophysical research communications. 298(4) |
| ISSN: | 0006-291X |
| Popis: | Two isoforms of the src-family tyrosine kinase p59fyn have been shown to arise through alternative splicing of exon 7 of the fyn gene. These isoforms have been designated fynT, expressed in hematopoietic cells, and fynB, expressed in the brain. Here, we describe a novel isoform, fynDelta7, in which exon 7 is absent. FynDelta7 mRNA transcripts have been identified in peripheral blood mononuclear cells from all individuals tested thus far and semi-quantitative RT-PCR indicates that this fyn transcript is expressed at near wild-type levels. Transcripts coding for the fynDelta7 isoform can be detected in purified B cells, T cells, NK cells, and monocytes, indicating that it is not lineage specific. We further show that the message isolated is functional using an in vitro expression system and by its expression in COS cells. |
| Databáze: | OpenAIRE |
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