252Cf plasma-desorption mass spectrometry of lipid a fromEnterobacter agglomerans
| Autor: | Connie David, Richard B. Cole, Linda N. Domelsmith, Roger A. Laine, J. C. Promé, Anthony J. DeLucca |
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| Rok vydání: | 1992 |
| Předmět: |
Byssinosis
Molecular Structure biology Chemistry Californium Molecular Sequence Data Organic Chemistry Enterobacter Mass spectrometry biology.organism_classification medicine.disease Enterobacteriaceae Mass Spectrometry Analytical Chemistry Lipid A Carbohydrate Sequence Biochemistry Desorption Mass spectrum medicine lipids (amino acids peptides and proteins) Spectroscopy Bacteria Myristoylation |
| Zdroj: | Rapid Communications in Mass Spectrometry. 6:616-622 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.1290061006 |
| Popis: | Endotoxins from gram-negative bacteria are believed to be causative agents of byssinosis, an occupational pulmonary disease associated with exposure to cotton dust in textile mills. Lipid A preparations from Enterobacter agglomerans, a gram-negative bacterium commonly found in cotton and cotton dust, have been analyzed using plasma-desorption mass spectrometry. Results indicate the existence of at least two lipid A types which differ only by the presence of an additional oxygen atom whose position has been localized to the acyloxyacyl ester-linked side-chain of the distal portion of the molecule. The lower molecular weight compound of the two structures has the same molecular weight and presumably the same empirical formula as a well-characterized lipid A from Salmonella minnesota. The mass spectra of lipid A compounds obtained from S. minnesota and E. agglomerans show strong similarities. Palmitoyl, hydroxymyristoyl, myristoyl, and lauroyl side-chains which are known to be present in the former are inferred from spectral evidence to be present in the latter. |
| Databáze: | OpenAIRE |
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