Fast‐forward genetics by radiation hybrids to saturate the locus regulating nuclear–cytoplasmic compatibility in Triticum
| Autor: | Filippo M. Bassi, Mohamed Mergoum, Shahryar F. Kianian, Rhoderissa Dizon, Farhad Ghavami, Kerrie L. Forrest, Yi Wang, Monika Michalak de Jiménez, Yong Q. Gu, Steven W. Meinhardt, Stephan Kong, Matthew J. Hayden |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cytoplasm Candidate gene Locus (genetics) Plant Science Genes Plant Evolution Molecular 03 medical and health sciences Gene Expression Regulation Plant wheat Aegilops tauschii Radiation hybrid mapping ORFS BulkSeq Gene Alleles Triticum Research Articles Cell Nucleus 2. Zero hunger Genetics Radiation Hybrid Mapping Bacterial artificial chromosome biology Contig synteny fast‐forward genetics Genetic Variation food and beverages Physical Chromosome Mapping biology.organism_classification species cytoplasm specific 030104 developmental biology speciation Agronomy and Crop Science Research Article Biotechnology |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 1467-7644 |
| DOI: | 10.1111/pbi.12532 |
| Popis: | Summary The nuclear‐encoded species cytoplasm specific (scs) genes control nuclear–cytoplasmic compatibility in wheat (genus Triticum). Alloplasmic cells, which have nucleus and cytoplasm derived from different species, produce vigorous and vital organisms only when the correct version of scs is present in their nucleus. In this study, bulks of in vivo radiation hybrids segregating for the scs phenotype have been genotyped by sequencing with over 1.9 million markers. The high marker saturation obtained for a critical region of chromosome 1D allowed identification of 3318 reads that mapped in close proximity of the scs. A novel in silico approach was deployed to extend these short reads to sequences of up to 70 Kb in length and identify candidate open reading frames (ORFs). Markers were developed to anchor the short contigs containing ORFs to a radiation hybrid map of 650 individuals with resolution of 288 Kb. The region containing the scs locus was narrowed to a single Bacterial Artificial Chromosome (BAC) contig of Aegilops tauschii. Its sequencing and assembly by nano‐mapping allowed rapid identification of a rhomboid gene as the only ORF existing within the refined scs locus. Resequencing of this gene from multiple germplasm sources identified a single nucleotide mutation, which gives rise to a functional amino acid change. Gene expression characterization revealed that an active copy of this rhomboid exists on all homoeologous chromosomes of wheat, and depending on the specific cytoplasm each copy is preferentially expressed. Therefore, a new methodology was applied to unique genetic stocks to rapidly identify a strong candidate gene for the control of nuclear–cytoplasmic compatibility in wheat. |
| Databáze: | OpenAIRE |
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