Fast, sensitive and selective liquid chromatographic–tandem mass spectrometric determination of tumor-promoting diterpene esters
| ISSN: | 0021-9673 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b65be181820f41e5ef4d4ec0ef7300f https://doi.org/10.1016/s0021-9673(99)00728-1 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....1b65be181820f41e5ef4d4ec0ef7300f |
| Autor: | S Achatz, Antonius Kettrup, G Vogg, H Sandermann |
| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Chemistry Organic Chemistry Extraction (chemistry) Reproducibility of Results food and beverages General Medicine Fractionation Mass spectrometry Sensitivity and Specificity Biochemistry High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Ion chemistry.chemical_compound Phorbol Esters Carcinogens Phorbol Diterpene Selectivity Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 855:563-573 |
| ISSN: | 0021-9673 |
| Popis: | A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed to detect tumor-promoting diterpene esters of the tigliane and ingenane types within plant extracts. Fractionation on a C18 high-performance liquid chromatography (HPLC) column was followed by MS-MS-multiple reaction monitoring (MRM) using the precursor-->product ion pairs of m/z 311-->293 and 293-->265 for phorbol esters. The ion pairs m/z 313-->295 and 295-->267 were used for ingenol and deoxyphorbol esters. In a second run, the characteristic ions at m/z 311 and 313 were followed in precursor ion scan mode. These quasi-molecular ions were utilized to obtain full scan spectra of the compounds in product ion scan mode. Due to its selectivity, the present on-line method can be applied for plant cultivar selection and plant product control without time-consuming extraction procedures and complex bioassays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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