Fluorimetric method for the detection of anti-DNA antibodies in serum
| Autor: | Bevra H. Hahn, Brian Mandell, Jagdish Mehta, Alberto Gallacher, Isaias Spilberg |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Radioimmunoassay
Antigen-Antibody Complex chemistry.chemical_compound Glomerulonephritis Antibody Specificity Fluorometer Humans Immune Complex Diseases Lupus Erythematosus Systemic Medicine Fluorometry Reactivity (chemistry) Fluorescein Fluorescein isothiocyanate Anti dna Chromatography biology business.industry DNA General Medicine FARR assay Fluoresceins chemistry Antibodies Antinuclear biology.protein Antibody business |
| Zdroj: | The American Journal of the Medical Sciences. 272:83-88 |
| ISSN: | 0002-9629 |
| DOI: | 10.1097/00000441-197607000-00010 |
| Popis: | A method for the detection of native anti-DNA antibodies in serum is described. The method is based on the reactivity of fluorescein isothiocyanate with DNA, forming a complex capable of combining with anti-DNA antibodies. The fluorescein content of the precipitated fluorescein-DNA anti-DNA complex is then measured in a fluorometer. The assay is accurate, highly reproducible, and inexpensive to perform. Comparative studies performed with the Farr assay show the fluorimetric method to be more sensitive in detecting anti-DNA antibodies in the serum of SLE patients. |
| Databáze: | OpenAIRE |
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