Simultaneous determination of amodiaquine and its active metabolite in human blood by ion-pair liquid chromatography-tandem mass spectrometry
| Autor: | Pan Deng, Xiaojian Dai, Dafang Zhong, Xiaoyan Chen |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Chromatography Acetonitriles Hydrochloride Metabolite Clinical Biochemistry Selected reaction monitoring Amodiaquine Atmospheric-pressure chemical ionization Cell Biology General Medicine Biochemistry Sensitivity and Specificity Analytical Chemistry chemistry.chemical_compound chemistry Drug Stability Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Reagent Humans Acetonitrile Active metabolite Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 860(1) |
| ISSN: | 1570-0232 |
| Popis: | A sensitive and selective ion-pair liquid chromatography-tandem mass spectrometric method (IP-LC-MS/MS) for the simultaneous determination of amodiaquine (AQ) and its active metabolite, N-desethylamodiaquine (AQm), in human blood has been developed and validated. Pentafluoropropionic acid (PFPA) was applied as ion-pairing reagent in reversed-phase chromatographic separation. The effects of PFPA concentrations and the volume fraction of acetonitrile in the mobile phase on the retention of analytes were investigated on a Venusil MP-C(18) column, and the mobile phase was finally optimized as acetonitrile:water (23:77, v/v) with 0.0667% PFPA in the aqueous phase. The results proved that PFPA as an ion-pairing reagent could provide desirable chromatographic performance in the IP-LC-MS/MS determination of 4-aminoquinoline compounds. Blood samples were protein precipitated with acetonitrile using hydroxychloroquine (OHCQ) as the internal standard. The detection was carried out in multiple reaction monitoring (MRM) mode via positive atmospheric pressure chemical ionization (APCI) interface. The lower limits of quantification were established at 0.150 and 1.50 ng/mL for AQ and AQm, respectively. The validated IP-LC-MS/MS method was applied to a clinical pharmacokinetic study of AQ and AQm in human blood after an oral administration of 600 mg AQ hydrochloride (45 9mg base). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect