Development of an ionic liquid-based ultrasonic-assisted liquid–liquid microextraction method for sensitive determination of biogenic amines: Application to the analysis of octopamine, tyramine and phenethylamine in beer samples
| Autor: | Yan-Ming Liu, Chun-Xue Jin, Cai-Yun Wei, Jing Li, Ke-Jing Huang, Shi-Lin Song |
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| Rok vydání: | 2011 |
| Předmět: |
Biogenic Amines
Phenethylamine Acetonitriles Clinical Biochemistry Ionic Liquids Tyramine Centrifugation Chemical Fractionation Biochemistry High-performance liquid chromatography Fluorescence spectroscopy Analytical Chemistry Sonication chemistry.chemical_compound Biogenic amine Phenethylamines Sample preparation Derivatization Octopamine Chromatography High Pressure Liquid Detection limit chemistry.chemical_classification Chromatography Temperature Beer Cell Biology General Medicine Hydrogen-Ion Concentration Spectrometry Fluorescence chemistry Ionic liquid Food Analysis |
| Zdroj: | Journal of Chromatography B. 879:579-584 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2011.01.018 |
| Popis: | A simple and efficient method, ionic liquid-based ultrasound-assisted liquid–liquid microextraction, has been developed for the determination of three biogenic amines including octopamine (OCT), tyramine (TYR) and phenethylamine (PHE). Fluorescence probe 2, 6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester was applied for derivatization of biogenic amines and high-performance liquid chromatography coupled with fluorescence detection was used for the determination of the derivatives. The factors affecting the extraction efficiency, such as the type and volume of ionic liquid, ultrasonication time and centrifugation time have been investigated in detail. Under the optimum conditions, linearity of the method was observed in the range of 0.5–50 μg mL−1 for OCT and TYR, and 0.025–2.5 μg mL−1 for PHE, respectively, with correlation coefficients (γ) > 0.996. The limits of detection ranged from 0.25–50 ng mL−1 (S/N = 3). The spiked recoveries of three target compounds in beer samples were in the range of 90.2–114%. As a result, this method has been successfully applied for the sensitive determination of OCT, TYR and PHE in beer samples. |
| Databáze: | OpenAIRE |
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