Regulation of Myogenic Activity by Substrate and Electrical Stimulation In Vitro
Autor: | Natalia Ziemkiewicz, Sara Vendrell-Gonzalez, Anjali Patel, Madison Marcinczyk, Koyal Garg, Jonathan S. Fisher, Muhamed Talovic, Gabriel Haas |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
collagen
0206 medical engineering lcsh:Medicine 02 engineering and technology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound laminin medicine Myocyte Original Research Article electrical stimulation lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Myogenesis Regeneration (biology) myoblasts lcsh:R Skeletal muscle musculoskeletal system 020601 biomedical engineering Cell biology Vascular endothelial growth factor medicine.anatomical_structure chemistry lcsh:Biology (General) Desmin Stem cell C2C12 |
Zdroj: | BioResearch Open Access, Vol 8, Iss 1, Pp 129-138 (2019) BioResearch Open Access |
ISSN: | 2164-7860 |
Popis: | Skeletal muscle has a remarkable regenerative capacity in response to mild injury. However, when muscle is severely injured, muscle regeneration is impaired due to the loss of muscle-resident stem cells, known as satellite cells. Fibrotic tissue, primarily comprising collagen I (COL), is deposited with this critical loss of muscle. In recent studies, supplementation of laminin (LM)-111 has been shown to improve skeletal muscle regeneration in several models of disease and injury. Additionally, electrical stimulation (E-stim) has been investigated as a possible rehabilitation therapy to improve muscle's functional recovery. This study investigated the role of E-stim and substrate in regulating myogenic response. C2C12 myoblasts were allowed to differentiate into myotubes on COL- and LM-coated polydimethylsiloxane molds. The myotubes were subjected to E-stim and compared with nonstimulated controls. While E-stim resulted in increased myogenic activity, irrespective of substrate, LM supported increased proliferation and uniform distribution of C2C12 myoblasts. In addition, C2C12 myoblasts cultured on LM showed higher Sirtuin 1, mammalian target of rapamycin, desmin, nitric oxide, and vascular endothelial growth factor expression. Taken together, these results suggest that an LM substrate is more conducive to myoblast growth and differentiation in response to E-stim in vitro. |
Databáze: | OpenAIRE |
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