Isolation techniques for anthocyanidin 3,5-diglucosides and their related chemicals using supramolecules technique, and two solid-phase extraction cartridges
| Autor: | Yoriko Koi, Mayumi Sadaoka, Hirotoshi Tamura, Risa Arakawa, Takayuki Asada, Fengxian Zhu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Flavonoids
Chromatography Chemistry Solid Phase Extraction Organic Chemistry Cyanidin Protocyanin General Medicine Perilla Biochemistry Analytical Chemistry Anthocyanins chemistry.chemical_compound Pigment Hydrolysis Glucosides visual_art visual_art.visual_art_medium Solid phase extraction Delphinidin Anthocyanidin |
| Zdroj: | Journal of Chromatography A. 1351:21-28 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2014.05.039 |
| Popis: | Isolation techniques for anthocyanidin 3,5-diglucosides and their derivatives from three types of supramolecular pigments were newly developed using two SPE (Discovery DPA-6S and DSC-SCX) cartridges. Initially, malonylawobanin from commelinin supramolecular complex yielded 79.5% with 85.7% purity using DPA-6S, while DSC-SCX gave a rather lower yield (62.5%) of malonylawobanin with high purity (90.8%). Delphinidin 3,5-diglucoside and awobanin from acidic hydrolysis of commelinin were easily separated using DPA-6S. Secondary, artificial Perilla supramolecules (reconstructed from Perilla crude pigment, flavocommelin and Mg(2+)) result in a high purity of a malonylshisonin and shisonin mixture (96.1%) with DSC-SCX. Finally, succinylcyanin, which is a component of protocyanin, yielded 64.3% with 86.1% purity using DPA-6S and 51.8% with 74.1% purity using DSC-SCX. Moreover, cyanidin 3,5-diglucoside yielded 43.4% with 97.0% purity using DPA-6S after partial acidic hydrolysis of protocyanin. |
| Databáze: | OpenAIRE |
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