Intraischemic Modest Hypothermia Does Not Prevent Onset of Locomotor Inactivity After Transient Forebrain Ischemia in Rats
| Autor: | Yoko Masaki, Go Nagasaki, Takashi Horiguchi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Behavior Animal business.industry Ischemia Rodent model Motor Activity Hypothermia Critical Care and Intensive Care Medicine medicine.disease Forebrain ischemia Neuroprotection Brain Ischemia Rats Rats Sprague-Dawley Prosencephalon Anesthesiology and Pain Medicine Hypothermia Induced Anesthesia medicine Animals medicine.symptom Maze Learning business CA1 Region Hippocampal |
| Zdroj: | Therapeutic Hypothermia and Temperature Management. 9:197-203 |
| ISSN: | 2153-7933 2153-7658 |
| Popis: | Although modest hypothermia of 35°C has been demonstrated to provide histological neuroprotection in a rodent model of cerebral ischemia, the long-term behavioral outcome is still not clear. This study was designed to investigate whether modest hypothermia of 35°C provides sustained histological and behavioral neuroprotection following transient forebrain ischemia in rats. Male Sprague-Dawley rats were randomly assigned to one of three groups: sham, control, and modest hypothermia group. Each group contained eight rats. Ten-minute transient forebrain ischemia was produced by bilateral carotid artery occlusion plus hemorrhagic hypotension (mean arterial pressure = 40 mmHg). The hypothermic group was cooled to 35°C in preischemic period, and the cooling was continued for 1 hour postischemia. To evaluate behavioral outcome, spontaneous alternation behavior and locomotor activity were assessed using Y-maze test on a weekly basis. The rats were sacrificed after 28 days, and the number of intact neurons per 1 mm in the hippocampal CA1 subfield was counted microscopically. There was significant difference between the control [19(24.5)/mm: median (interquartile range)] and hypothermia groups [116(24)/mm |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|