Increased native chemiluminescence in granulocytes isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis
| Autor: | K. Sonntag, Klaus Arnold, H. Sauer, Holm Häntzschel, Juergen Arnhold |
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| Rok vydání: | 1994 |
| Předmět: |
Azides
Neutrophils Radical Biophysics Deferoxamine In Vitro Techniques law.invention Arthritis Rheumatoid chemistry.chemical_compound Reference Values law Synovial Fluid medicine Humans Synovial fluid Mannitol Sodium Azide Chemiluminescence biology Superoxide Dismutase Thiourea Glutathione Catalase medicine.disease Molecular biology chemistry Chemistry (miscellaneous) Rheumatoid arthritis Luminescent Measurements Immunology biology.protein Tetradecanoylphorbol Acetate Sodium azide |
| Zdroj: | Journal of Bioluminescence and Chemiluminescence. 9:79-86 |
| ISSN: | 1099-1271 0884-3996 |
| DOI: | 10.1002/bio.1170090207 |
| Popis: | Polymorphonuclear leukocytes (PMNs) isolated from peripheral blood and synovial fluid of patients with rheumatoid arthritis and from peripheral blood of volunteers were stimulated with 12-phorbol-13-myristate acetate (PMA). No significant differences in luminol-amplified chemiluminescence were found between different patients and control groups. However, two distinct patterns of native chemiluminescence were observed. Type I showed no, or only a small, increase in native chemiluminescence with integral counts over 30 min less than 3 x 10(5) cpm, and the majority of samples from volunteers were of this type. Type II was characterized by a burst of native chemiluminescence starting 8 to 15 min after cell stimulation. It was found in most PMN samples from patients with rheumatoid arthritis. Integral counts over 30 min were always higher than 10(6) cpm and as high as 10(8) cpm in some cases. A strong inhibition of the Type II native chemiluminescence was caused by desferal, catalase, thiourea, and glutathione. However, the luminol-amplified chemiluminescence remained unchanged or was only slightly decreased under the same experimental conditions. Sodium azide strongly inhibited both kinds of luminescence. Hydroxyl radicals, formed in a Fenton reaction, may be important intermediates in the Type II native chemiluminescence. |
| Databáze: | OpenAIRE |
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