The recombinant maize ribosome-inactivating protein transiently reduces viral load in SHIV89.6 infected Chinese Rhesus Macaques
| Autor: | Kam-Bo Wong, Wei Pang, Amanda Nga-Sze Mak, Ka-Yee Au, Sue Ka-Yee Law, Gao-Hong Zhang, Xuan Zhang, Rong-Hua Luo, Yong-Tang Zheng, Hong-Yi Zheng, Rui-Rui Wang, Liang-Min Gao, Pang-Chui Shaw, Ming-Xu Zhang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Health Toxicology and Mutagenesis Ribosome Inactivating Proteins Simian Acquired Immunodeficiency Syndrome lcsh:Medicine Toxicology Macaque Zea mays Article law.invention Immune system law biology.animal maize RIP Protein biosynthesis Animals biology Ribosome-inactivating protein animal model lcsh:R Ribosomal RNA Viral Load anti-HIV Virology Macaca mulatta Recombinant Proteins Anti-Retroviral Agents Recombinant DNA Leukocytes Mononuclear Simian Immunodeficiency Virus Viral load Ex vivo |
| Zdroj: | Toxins Toxins, Vol 7, Iss 1, Pp 156-169 (2015) Volume 7 Issue 1 Pages 156-169 |
| ISSN: | 2072-6651 |
| Popis: | Ribosome inactivating proteins (RIPs) inhibit protein synthesis by depurinating the large ribosomal RNA and some are found to possess anti-human immunodeficiency virus (HIV) activity. Maize ribosome inactivating protein (RIP) has an internal inactivation loop which is proteolytically removed for full catalytic activity. Here, we showed that the recombinant active maize RIP protected chimeric simian-human immunodeficiency virus (SHIV) 89.6-infected macaque peripheral blood mononuclear cells from lysis ex vivo and transiently reduced plasma viral load in SHIV89.6-infected rhesus macaque model. No evidence of immune dysregulation and other obvious side-effects was found in the treated macaques. Our work demonstrates the potential development of maize RIP as an anti-HIV agent without impeding systemic immune functions. |
| Databáze: | OpenAIRE |
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