Novel Embryonic Genes Are Preferentially Expressed by Autonomously Replicating Rat Embryonic and Neointimal Smooth Muscle Cells
Autor: | Bryan E. Quinn, James K. Belknap, Philip E. Schwartz, Kurt R. Stenmark, Richard A. Majack, Peter M. Mourani, Nicole A. Grieshaber, Scott S. Grieshaber, Mary C.M. Weiser-Evans |
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Rok vydání: | 2000 |
Předmět: |
Male
Pathology medicine.medical_specialty Physiology Autonomously replicating sequence Gene Expression Neovascularization Physiologic Biology Muscle Smooth Vascular Rats Sprague-Dawley Complementary DNA Gene expression medicine Animals Cloning Molecular Gene Aorta Cell growth cDNA library Gene Expression Profiling Proteins Embryo Mammalian musculoskeletal system Phenotype Embryonic stem cell Rats Cell biology cardiovascular system Cardiology and Cardiovascular Medicine Cell Division |
Zdroj: | Circulation Research. 87:608-615 |
ISSN: | 1524-4571 0009-7330 |
DOI: | 10.1161/01.res.87.7.608 |
Popis: | Abstract —We sought to identify and characterize the expression pattern of genes expressed by smooth muscle cells (SMCs) during periods of self-driven replication during vascular development and after vascular injury. Primary screening of a rat embryonic aortic SMC–specific cDNA library was accomplished with an autonomous embryonic SMC–enriched, nonautonomous adult SMC–subtracted cDNA probe. Positive clones were rescreened in parallel with embryonic SMC–specific and adult SMC–specific cDNA probes. We identified 14 clones that hybridized only with the embryonic cDNA (“emb” clones), 11 of which did not share significant homology with sequences in any of the databases. Five of these novel emb genes (emb7, emb8, emb20, emb37, and emb41) were selectively and only transiently reexpressed in vivo by neointimal SMCs during periods of rapid replication. The emb8:embryonic growth–associated protein (EGAP), which was studied the most extensively, was expressed at high levels by cultured, autonomously replicating embryonic and neointimal SMCs but was detected only at low levels even in mitogenically stimulated adult SMCs. Finally, the administration of antisense EGAP oligonucleotides markedly attenuated embryonic and neointimal SMC replication rates. We suggest that autonomous replication of SMCs may be essential for normal vascular morphogenesis and for the vascular response to injury and that these newly identified “embryonic” genes may be part of the molecular machinery that drives this unique growth phenotype. |
Databáze: | OpenAIRE |
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