In situ identification of neuronal nitric oxide synthase (NOS-I) mRNA in mouse and rat skeletal muscle
| Autor: | Dieter Blottner, Gabriele Lück, Ilse Oberbäumer |
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| Rok vydání: | 1998 |
| Předmět: |
Transcription
Genetic Muscle Fibers Skeletal Nitric Oxide Synthase Type I In situ hybridization Biology Polymerase Chain Reaction Cell Line Mice Sarcolemma Myofibrils Gene expression medicine Animals Myocyte RNA Messenger Muscle Skeletal In Situ Hybridization DNA Primers Myogenesis General Neuroscience Skeletal muscle Cell Differentiation Molecular biology Rats Nitric oxide synthase medicine.anatomical_structure biology.protein Nitric Oxide Synthase DNA Probes Myofibril C2C12 |
| Zdroj: | Neuroscience Letters. 246:77-80 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(98)00237-7 |
| Popis: | Skeletal muscle provides a major source of the signaling molecule nitric oxide (NO) however in situ identification of NO-synthase (NOS) mRNA has not been verified. We have used NOS-I (neuronal NOS) probes prepared from plasmid DNA by reverse transcription-polymerase chain reaction (RT-PCR) to detect mRNA transcripts in skeletal muscle cells and myofibers of rat and mouse. Mouse C2C12 myoblasts and myotubes reveal strong cytosolic in situ hybridization (ISH) signals in vitro. In adult animals, ISH signals are detectable in striated myofibers at subsarcolemmal and perinuclear regions whilst the myofibrillar compartment is devoid of signals. Expression of NOS-I mRNA in fusion-competent myoblasts suggests that the NOS/NO system is of relevance to myogenic differentiation. Compartmentalization of NOS-I mRNA may reflect spatiofunctional actions between NOS message and protein and the putative subcellular NO targets. |
| Databáze: | OpenAIRE |
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