Dynamic Expression of Genes Involved in Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
| Autor: | E.M.A. van de Westerlo, T.H. van Kuppevelt, J. van der Vlag, Willeke F. Daamen, Elly M. M. Versteeg, P.J.E. Uijtdewilligen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell signaling Article Subject medicine.medical_treatment lcsh:Medicine General Biochemistry Genetics and Molecular Biology Glycosaminoglycan 03 medical and health sciences Mice All institutes and research themes of the Radboud University Medical Center medicine Animals Heparanase Gene Glycosaminoglycans Skin Regulation of gene expression General Immunology and Microbiology biology Microarray analysis techniques Chemistry Growth factor lcsh:R General Medicine Dermis Cell biology 030104 developmental biology Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] Proteoglycan Gene Expression Regulation biology.protein Female Proteoglycans |
| Zdroj: | BioMed Research International, Vol 2018 (2018) Biomed Research International, 2018, Biomed Research International, 2018, pp. |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Glycosaminoglycans are important for cell signaling and therefore for proper embryonic development and adult homeostasis. Expressions of genes involved in proteoglycan/glycosaminoglycan (GAG) metabolism and of genes coding for growth factors known to bind GAGs were analyzed during skin development by microarray analysis and real time quantitative PCR. GAG related genes were organized in six categories based on their role in GAG homeostasis, viz. (1) production of precursor molecules, (2) production of core proteins, (3) synthesis of the linkage region, (4) polymerization, (5) modification, and (6) degradation of the GAG chain. In all categories highly dynamic up- and downregulations were observed during skin development, including differential expression of GAG modifying isoenzymes, core proteins, and growth factors. In two mice models, one overexpressing heparanase and one lacking C5 epimerase, differential expression of only few genes was observed. Data show that during skin development a highly dynamic and complex expression of GAG-associated genes occurs. This likely reflects quantitative and qualitative changes in GAGs/proteoglycans, including structural fine tuning, which may be correlated with growth factor handling. |
| Databáze: | OpenAIRE |
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