A 39-kDa capsular protein is a major cross-protection factor as demonstrated by protection of chickens with a live attenuated Pasteurella multocida strain of P-1059
| Autor: | Chaisuree Suphavilai, Yasushi Kataoka, Terdsak Yano, Nattawooti Sthitmatee, Takuo Sawada, Kannikar Na Lampang |
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| Rok vydání: | 2013 |
| Předmět: |
Attenuated vaccine
General Veterinary Strain (chemistry) Cross Protection Pasteurella Infections Heterologous Enzyme-Linked Immunosorbent Assay Biology biology.organism_classification Virology Immune system Species Specificity Immunity Post vaccination Bacterial Vaccines Animals Fowl cholera Pasteurella multocida Chickens Poultry Diseases Bacterial Outer Membrane Proteins |
| Zdroj: | The Journal of veterinary medical science. 75(7) |
| ISSN: | 1347-7439 |
| Popis: | The aim of this study was to show that a 39-kDa protein or OmpH of Pasteurella multocida strain P-1059 is essential for cross protection. Strain PBA322, a thinly capsulated strain of P. multocida strain P-1059, was used as a live vaccine in chickens. Strain PBA322 is a thinly capsulated strain in comparison with the parental strain P-1059. Chickens were vaccinated by single injection and then challenge-exposed with strains P-1059 or X-73 at two weeks post vaccination. Moreover, immune responses were also evaluated for both humoral and cellular immune response by ELISA and lymphocyte proliferation assay, respectively. The results showed that the live vaccine induced efficient immunity to protect chickens from challenge-exposure to the parent strain, but that the heterologous protection was poor. We concluded that the 39-kDa protein is essential for cross protection. |
| Databáze: | OpenAIRE |
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