Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells
| Autor: | Ece Şimşek, Esra Aydemir, Aylin Fidan Korcum, Kayahan Fiskin |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Angiogenesis Angiogenesis Inhibitors Breast Neoplasms Biochemistry 03 medical and health sciences Enzyme activator ADAM10 Protein 0302 clinical medicine Cell Line Tumor Genetics medicine Humans Molecular Biology Neprilysin biology Oncogene Neovascularization Pathologic Radiotherapy Cancer medicine.disease Enzyme assay Endostatins Enzyme Activation 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research biology.protein Molecular Medicine Female Endostatin |
| Zdroj: | Molecular medicine reports. 14(3) |
| ISSN: | 1791-3004 |
| Popis: | Angiogenesis, the formation of new blood vessels, is regarded as a key cancer cell property. Endostatin (ES) is a potential antiangiogenic agent and it may be useful when implemented in combination with other cancer therapeutic strategies. The present study investigated the in vitro effects of ES, radiotherapy (RT) or combination therapy (ES + RT) on two important proteases, a disintegrin and metalloproteinase domain‑containing protein 10 (ADAM10) and neprilysin (NEP) in 4T1 mouse breast cancer cells and the more metastatic phenotype of 4THMpc breast cancer cells. 4T1 and 4THMpc cells were treated with recombinant murine ES (4 µg/ml) alone, RT (45 Gy) alone or with ES + RT. ADAM10 enzyme activity was determined using a tumor necrosis factor‑α converting enzyme (α‑secretase) activity assay kit, and NEP enzyme activity was measured with a fluorometric assay based on the generation of free dansyl‑D‑Ala‑Gly from N-dansyl-Ala-Gly-D-nitro-Phe-Gly, the substrate of NEP. Western blotting analysis was performed to determine whether the altered enzyme activity levels of the two cell lines occurred due to changes in expression level. These data indicate that ES independently potentiates the activity of ADAM10 and NEP enzymes in 4T1 and 4THMpc breast cancer cells. |
| Databáze: | OpenAIRE |
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