Convergent and General One-Step DNA-Catalyzed Synthesis of Multiply Branched DNA
| Autor: | Timothy P. Mui, Scott K. Silverman |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
DNA ligase DNA clamp Base Sequence DNA synthesis biology Chemistry Base pair Stereochemistry Organic Chemistry Deoxyribozyme DNA replication DNA DNA Catalytic Processivity Biochemistry Article Catalysis biology.protein Physical and Theoretical Chemistry DNA polymerase I |
| Zdroj: | Organic Letters. 10:4417-4420 |
| ISSN: | 1523-7052 1523-7060 |
| Popis: | We report a deoxyribozyme (DNA enzyme) that catalyzes the convergent and general synthesis of branched DNA. The 15HA9 deoxyribozyme mediates nucleophilic attack of the 2'-hydroxyl group of a ribonucleotide embedded within one DNA substrate into a 5'-adenylate of the second DNA substrate. This approach can be used to synthesize multiply branched DNA with a wide range of DNA sequences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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