Increased M1 Macrophages Infiltration Is Associated with Thrombogenesis in Rheumatic Mitral Stenosis Patients with Atrial Fibrillation
| Autor: | Suhui Zhu, Jun Xie, Guixin He, Bingjian Wang, Biao Xu, Guannan Li, Wei Tan, Jianzhou Chen |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology Inflammasomes Interleukin-1beta lcsh:Medicine Apoptosis 030204 cardiovascular system & hematology Pathology and Laboratory Medicine Biochemistry White Blood Cells 0302 clinical medicine Animal Cells Atrial Fibrillation Medicine and Health Sciences Mitral Valve Stenosis Myocytes Cardiac Cardiac Atria lcsh:Science Immune Response Stenosis Immune System Proteins Multidisciplinary Cell Death Heart Atrial fibrillation Middle Aged M2 Macrophage Thrombosis Cell Processes cardiovascular system Cardiology Female Cellular Types Anatomy medicine.symptom Arrhythmia Research Article Adult medicine.medical_specialty Patients Immune Cells Immunology Inflammation Proinflammatory cytokine 03 medical and health sciences Signs and Symptoms Mitral valve stenosis Internal medicine NLR Family Pyrin Domain-Containing 3 Protein medicine Humans cardiovascular diseases Thrombus Blood Cells business.industry Macrophages lcsh:R Rheumatic Heart Disease Biology and Life Sciences Proteins Cell Biology medicine.disease Health Care 030104 developmental biology Cardiovascular Anatomy lcsh:Q Carrier Proteins business |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0149910 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0149910 |
| Popis: | Atrial fibrillation (AF) is the most common arrhythmia. In patients with AF, the role of macrophage subsets in thrombogenesis is unclear. In the present study, we analyzed the role of M1 and M2 macrophages and related cytokines in thrombogenesis of AF. Immunohistochemistry, Western blot, and TUNEL assay were used to detect M1/M2 macrophage infiltration, the expression pattern of IL-1β and inflammasome components, and apoptosis of cardiomyocytes in 71 specimens obtained from the left atrial appendage of patients with rheumatic mitral stenosis (MS) with or without thrombosis. We demonstrated that proinflammatory M1 macrophages were predominant in the atrium of MS patients with AF and thrombus. NLRP3 inflammasomes and IL-1β, which are primarily functional in macrophages, were activated in those patients. We also showed that increased cell death was associated with thrombogenesis in MS patients. These data indicate that infiltration of M1 macrophages and over-activation of NLRP3 inflammasomes may play a role in progressive atrial inflammation and thrombogenesis in rheumatic mitral stenosis patients with AF. |
| Databáze: | OpenAIRE |
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