Bovine steroid 21-hydroxylase: regulation of biosynthesis
| Autor: | Takashi Okamura, Beverly S. Adler, Maliyakal E. John, Michael R. Waterman, Perrin C. White, Evan R. Simpson, Albert Dee, Manorama C. John |
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| Rok vydání: | 1986 |
| Předmět: |
Transcription
Genetic Steroid 21-Hydroxylase Swine Biology Biochemistry Mice Cytochrome P-450 Enzyme System Species Specificity Transcription (biology) Complementary DNA Sequence Homology Nucleic Acid Gene expression Animals Humans Amino Acid Sequence Cloning Molecular Gene Peptide sequence Cells Cultured Base Sequence cDNA library RNA Nucleic Acid Hybridization DNA DNA Restriction Enzymes Molecular biology Kinetics Organ Specificity Steroid Hydroxylases Adrenal Cortex Cattle |
| Zdroj: | Biochemistry. 25(10) |
| ISSN: | 0006-2960 |
| Popis: | A recombinant cDNA clone, PBC21-1, specific for bovine steroid 21-hydroxylase cytochrome P-450 (P-450C21) was identified in a bovine adrenocortical cDNA library, and this identity was confirmed by nucleotide sequencing which revealed significant amino acid homology (77%) with human P-450C21 cDNA. The pBC21-1 insert is 1.7 kilobases in length and includes a 1128 base pair region that encodes the C-terminal 376 amino acids of bovine P-450C21 as well as 535 base pairs of 3'-untranslated sequence. A novel feature of this insert is a 20 base pair intervening sequence near the 5' end, apparently the result of an aberrant splicing event. Northern blot analysis reveals that bovine P-450C21 is encoded by two transcripts, 2.3 and 2.0 kilobases in length which are detected in adrenal cortical RNA. Bovine liver, heart, kidney, and corpus luteum do not contain detectable P-450C21 transcripts. Regulation of P-450C21 gene expression by adrenocorticotropin was investigated with pBC21-1 and bovine adrenocortical cells in primary, monolayer culture. Treatment with ACTH or analogues of cAMP increases the steady-state levels of P-450C21 RNA in such cell cultures. In vitro transcription run-on assays suggest that this increase is, at least in part, due to the enhanced transcriptional activity of the P-450C21 gene. |
| Databáze: | OpenAIRE |
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