Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization
| Autor: | T. Hagedorn-Olsen, H.J. Riising, Øystein Angen, Tim Kåre Jensen, Mette Boye |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences Swine animal diseases Lameness Animal Arthritis In situ hybridization Metaphysis Sensitivity and Specificity Microbiology 0403 veterinary science 03 medical and health sciences Actinobacillus Infections RNA Ribosomal 16S medicine Animals Actinobacillus pleuropneumoniae In Situ Hybridization Fluorescence Swine Diseases Pleuropneumonia General Veterinary biology Osteomyelitis 04 agricultural and veterinary sciences respiratory system Ribosomal RNA biology.organism_classification medicine.disease respiratory tract diseases Hindlimb RNA Bacterial 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence Lameness Joints DNA Probes |
| Zdroj: | Veterinary pathology. 36(3) |
| ISSN: | 0300-9858 |
| Popis: | Necrotizing osteomyelitis and fibrinopuralent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular met-aphysis, thus demonstrating that well-known infections manifest new, unusual lesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|