Clostridium perfringens alpha toxin is produced in the intestines of broiler chicks inoculated with an alpha toxin mutant
| Autor: | Robert D. Glock, Gayatri Vedantam, J.G. Songer, Hien T. Trinh, Christine F. Coursodon, Michael Mallozzi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Clostridium perfringens
Mutant Bacterial Toxins Virulence Clostridium perfringens alpha toxin Biology medicine.disease_cause Microbiology Birds In vivo medicine Animals heterocyclic compounds Poultry Diseases Strain (chemistry) Histocytochemistry Calcium-Binding Proteins Broiler Wild type Enteritis Intestines Infectious Diseases Type C Phospholipases cardiovascular system Mutant Proteins Chickens |
| Zdroj: | Anaerobe. 16(6) |
| ISSN: | 1095-8274 |
| Popis: | Poultry necrotic enteritis (NE) is caused by specific strains of Clostridium perfringens, most of which are type A. The role of alpha toxin (CPA) in NE has been called into question by the finding that an engineered cpa mutant retains full virulence in vivo[9]. This is in contrast to the finding that immunization with CPA toxoids protects against NE. We confirmed the earlier findings, in that 14-day-old Cornish × Rock broiler chicks challenged with a cpa mutant developed lesions compatible with NE in >90% of birds inoculated with the mutant. However, CPA was detected in amounts ranging from 10 to >100 ng per g of gut contents and mucosa in birds inoculated with the cpa mutant, the wildtype strain from which the mutant was constructed, and our positive control strain. There was a direct relationship between lesion severity and amount of CPA detected (R = 0.89-0.99). These findings suggest that the role of CPA in pathogenesis of NE requires further investigation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect