Effects of prenatal Poly I:C exposure on global histone deacetylase (HDAC) and DNA methyltransferase (DNMT) activity in the mouse brain
| Autor: | Sylvia de Jonge, Waldtraud Stettinger, Aye Mu Myint, Daniel L.A. van den Hove, Peter Zill, K. Neumeier, Gunter Kenis, Harry W.M. Steinbusch, Yara Pujol Lopez |
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| Přispěvatelé: | Promovendi MHN, Psychiatrie & Neuropsychologie, RS: MHeNs - R2 - Mental Health, MUMC+: MA Niet Med Staf Psychiatrie (9), RS: MHeNs - R3 - Neuroscience |
| Rok vydání: | 2016 |
| Předmět: |
Male
Poly I:C 0301 basic medicine medicine.medical_specialty Mouse Offspring Biology DNA methyltransferase Article Histone Deacetylases Epigenesis Genetic 03 medical and health sciences 0302 clinical medicine Pregnancy HDAC Internal medicine Genetics medicine Animals DNA (Cytosine-5-)-Methyltransferases Molecular Biology Histone deacetylase 2 Brain General Medicine HDAC6 HDAC3 Molecular biology HDAC4 HDAC1 Mice Inbred C57BL Poly I-C 030104 developmental biology Endocrinology Prenatal Exposure Delayed Effects Female Epigenetics Histone deacetylase Infection 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology Reports, 43(7), 711-717. Springer Molecular Biology Reports |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-016-4006-y |
| Popis: | The aim of our study was to investigate the brain-specific epigenetic effects on global enzymatic histone deacetylase (HDAC) and DNA methyltransferase (DNMT) activity after prenatal exposure to maternal immune challenge by polyinosinic:polycytidylic acid (Poly I:C) at gestational day (GD) 17 in C57BL/6JRccHsd mouse offspring. Pregnant mice were randomly divided into 2 groups, receiving either 5 mg/kg Poly I:C or phosphate buffered saline (PBS) intravenously at GD 17. Subsequently, the effects on whole brain enzymatic HDAC and DNMT activity and the protein levels of various HDAC isoforms were assessed in the offspring. Overall, a significant sex x treatment interaction effect was observed after prenatal exposure to maternal immune challenge by Poly I:C, indicative of increased global HDAC activity particularly in female offspring from mothers injected with Poly I:C when compared to controls. Results on the levels of specific HDAC isoforms suggested that neither differences in the levels of HDAC1, HDAC2, HDAC3, HDAC4 or HDAC6 could explain the increased global HDAC activity observed in female Poly I:C offspring. In conclusion, we show that Poly I:C administration to pregnant mice alters global brain HDAC, but not DNMT activity in adult offspring, whereas it is still unclear which specific HDAC(s) mediate(s) this effect. These results indicate the necessity for further research on the epigenetic effects of Poly I:C. |
| Databáze: | OpenAIRE |
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