In vitro proliferation and differentiation of human mesenchymal stem cells on hydroxyapatite versus human demineralised bone matrix with and without osteogenic protein-1
| Autor: | Amit Bhalla, Eleftherios Tsiridis, Zakareya Gamie, Manolis Heliotis, N Gurav, Sanjukta Deb, Lucy DiSilvio, Zubier Ali |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Bone Morphogenetic Protein 7 Clinical Biochemistry Bone Matrix Bone matrix Drug Discovery Humans Cells Cultured Cell Proliferation Pharmacology Bone Demineralization Technique Chemistry Mesenchymal stem cell dBm Cell Differentiation Mesenchymal Stem Cells Anatomy Alkaline Phosphatase Molecular biology In vitro Resorption Bone morphogenetic protein 7 Durapatite Microscopy Electron Scanning Alkaline phosphatase Stem cell |
| Zdroj: | Expert opinion on biological therapy. 9(1) |
| ISSN: | 1744-7682 |
| Popis: | Allografting is currently used in lower limb reconstruction surgery. Demineralised bone matrix (DBM) is more osteoinductive compared with allografts but lacks mechanical strength. Osteogenic protein-1 (OP-1) can improve the osteoinductivity of the allograft, however recent reports indicate significant allograft resorption when it is combined with OP-1.Our hypothesis was that hydroxyapatite (HA) with human-mesenchymal stem cells (h-MSCs) and OP-1 (HA+h-MSCs+OP-1) has similar osteoinductive properties to human-DBM+h-MSCs. The objective was to evaluate h-MSC proliferation (by tritiated thymidine incorporation, total DNA Hoechst 33258 and scanning electron microscopy) and osteogenic differentiation (from alkaline phosphatase activity) in human demineralised bone matrix (h-DBM) and HA, with or without OP-1.H-MSC proliferation on HA+OP-1 was significantly higher compared with that on HA at all time points (p0.05) and compared with DBM alone [day 1, (198.4 vs 95.4) p = 0.042; day 14 (286.1 vs 119.9), p0.001]. H-MSC proliferation was higher in DBM+OP-1, at all time points compared with HA+OP-1 but the difference was not statistically significant (p0.05). H-MSC differentiation was significantly higher in HA+OP-1 compared with HA (p0.05) but not significantly different from diffferentiation on DBM alone (p0.05). Differentiation was significantly higher on DBM+OP-1 at all time points compared with HA (p0.05) and with HA+OP-1 [day 1, (21.1 vs 10.1) (p = 0.03); day 7 (39.4 vs 7.1) (p0.01); day 14 (40.2 vs 14.4) (p0.001)].When HA+h-MSCs is combined with OP-1 in vitro its osteogenic potential is similar to that of DBM+h-MSCs alone which may be adequate for non-weight-bearing applications. Mechanical testing however is of great importance for weight-bearing applications and the in vivo testing of the composite graft HA+h-MSCs+OP-1 vs DBM+h-MSCs is recommended. |
| Databáze: | OpenAIRE |
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