Intrastriatal injection of hypoxanthine alters striatal ectonucleotidase activities: A time-dependent effect
| Autor: | Carlos Alexandre Netto, Giana de Paula Cognato, João José Freitas Sarkis, Angela T. S. Wyse, Carla Denise Bonan, Janaína Kolling, Denise Barbosa Ramos, Maurício Reis Bogo, Caren Serra Bavaresco, Fabria Chiarani |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Phosphoric monoester hydrolases Ascorbic Acid Striatum medicine.disease_cause Thiobarbituric Acid Reactive Substances chemistry.chemical_compound Antigens CD nucleotidases Internal medicine Nucleotidase medicine TBARS Animals Vitamin E Ectonucleotidase Pyrophosphatases Rats Wistar 5'-Nucleotidase Molecular Biology Hypoxanthine Adenosine Triphosphatases Nucleotides Hydrolysis General Neuroscience Apyrase Vitamins Corpus Striatum Pathophysiology Rats Endocrinology chemistry adenosine Neurology (clinical) Oxidative stress Central Nervous System Agents Developmental Biology |
| Zdroj: | Repositório Institucional PUCRS Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2008.08.015 |
| Popis: | The aim of this study was to investigate the effects of intrastriatal injection of hypoxanthine on ectonucleotidase (E-NTPDases and ecto-5′-nucleotidase) activities and expressions in the striatum of rats. The effect of pre-treatment with vitamins E and C on the effects elicited by this oxypurine on enzymatic activities and on thiobarbituric reactive substances (TBARS) was also investigated. The effect of pre-incubation with hypoxanthine on nucleotide hydrolysis in striatum homogenate was also determined. Adult Wistar rats were divided into (1) control and (2) hypoxanthine-injected groups. For ectonucleotidase activity determination, the animals were sacrificed at 30 min, 24 h and 7 days after drug infusion. For the evaluation of the expression of NTPDase 1–3 and also ecto-5′-nucleotidase, TBARS assay and the influence of the pre-treatment with vitamins on ectonucleotidase activities, the animals were sacrificed 24 h after hypoxanthine infusion. Results show that hypoxanthine infusion significantly inhibited ectonucleotidase activities and increased TBARS only 24 h after administration. Pre-treatment with vitamins was able to prevent these effects. Moreover, ecto-5′-nucleotidase expression was increased (80%) at 24 h after hypoxanthine infusion. We suggest that these hypoxanthine-induced biochemical modifications could, at least in part, participate in the pathophysiology of Lesch Nyhan disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect