Quality control of ion torrent sequencing library
| Autor: | Valentina Pileczki, Cornelia Braicu, Emil Puscas, Laura-Ancuţa Pop, Roxana Cojocneanu-Petric, Patriciu Achimas-Cadariu, Ioana Berindan-Neagoe |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Electrophoresis
Quality Control Cancer Research Computer science media_common.quotation_subject Sequencing data High-Throughput Nucleotide Sequencing Breast Neoplasms General Medicine Computational biology Ion semiconductor sequencing Real-Time Polymerase Chain Reaction Response to treatment Oncology Spectrophotometry Genetics Humans Quality (business) Female media_common Gene Library |
| Zdroj: | Cancer biomarkers : section A of Disease markers. 14(2-3) |
| ISSN: | 1875-8592 |
| Popis: | Next-generation sequencing (NSG) is an important method for gathering large amounts of sequencing data for different types of applications regarding the diagnosis and response to treatment of different diseases. An important step in the NGS process is the quality control of sequencing libraries, which can influence the yield and efficiency of the sequencing run. This study evaluated two different methods for library quality control, Agilent Bioanalyzer and qPCR, and showed that both methods can be used. However, as is the case with any analytical method, they have their limitations. The Agilent Bioanalyzer quantifies only the high quality libraries, but it underestimates their concentration, while qPCR also quantifies lower quality libraries, but it overestimates their concentration. |
| Databáze: | OpenAIRE |
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