The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl− channel

Autor:	Canhui Li, Paul Linsdell, Christine E. Bear, Yoshiro Sohma, Bakhrom K. Berdiev, Toby S. Scott-Ward, Xiandi Gong, Ilana Kogan, Hongyu Li, Michael A. Gray, Jeng-Haur Chen, Zhiwei Cai, Tzyh Chang Hwang, Mohabir Ramjeesingh, David N. Sheppard, Jyoti Gupta, Dale J. Benos, Iskander I. Ismailov, Martin J. Hug
Rok vydání:	2004
Předmět:	Pulmonary and Respiratory Medicine congenital hereditary and neonatal diseases and abnormalities Patch-Clamp Techniques Lipid Bilayers Cystic Fibrosis Transmembrane Conductance Regulator ATP-binding cassette transporter Gating Chloride channel activity Channel regulation Single-channel recording Whole-cell recording Medicine Humans Patch clamp Pediatrics Perinatology and Child Health Lipid bilayer Membrane potential Anion permeation biology business.industry Cystic fibrosis transmembrane conductance regulator Biochemistry Cyclic nucleotide-binding domain Pediatrics Perinatology and Child Health biology.protein Biophysics Channel gating business Ion Channel Gating
Zdroj:	Journal of Cystic Fibrosis. 3:101-108
ISSN:	1569-1993
DOI:	10.1016/j.jcf.2004.05.046
Popis:	Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl− channel with special emphasis on its permeation, conduction and gating properties.
Databáze:	OpenAIRE
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