Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study

Autor: Staffan Persson, Uroš Rot, Giovanni B. Frisoni, María Salud García-Ayllón, Hanne Struyfs, Inês Baldeiras, André Janeiro, Julia T. Tanassi, Davide Chiasserini, Andreja Emeršič, Norbert Zilka, Maria João Leitão, Eugeen Vanmechelen, Tormod Fladby, Luisella Bocchio Chiavetto, Henrik Zetterberg, Niels Kruse, Justyna M.C. Bahl, H. Bea Kuiperij, Javier Sáez-Valero, Madalena Martins, Branislav Kovacech, Erden Eren, Hanne M. Møllergård, Daniel Alcolea, Annika Öhrfelt, Alberto Lleó, Catarina R. Oliveira, Mafalda Matos, Marcel M. Verbeek, Lucilla Parnetti, Magda Tsolaki, Kaj Blennow, Brit Mollenhauer, Niels H. H. Heegaard, Elisabetta Capello, Flavio Nobili, Sebastiaan Engelborghs, Olymbia Gkatzima, Sermin Genc, Peggy Taylor
Přispěvatelé: Repositório da Universidade de Lisboa, Clinical sciences, Neurology
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
Aging
International Cooperation
Enzyme-Linked Immunosorbent Assay
Alpha-synuclein Cerebrospinal fluid ELISA Validation Biomarker PARKINSONS-DISEASE LEWY BODIES ALZHEIMERS-DISEASE ELEVATED LEVELS CSF DEMENTIA BIOMARKERS RECOMMENDATIONS QUANTIFICATION DISCRIMINATE
Alpha-synuclein
ddc:616.89
chemistry.chemical_compound
Cerebrospinal fluid
Alpha-synuclein
Biomarker
Cerebrospinal fluid
ELISA
Validation
Validation
Humans
Medicine
Biomarkers/cerebrospinal fluid
Biology
Medicine(all)
Parkinson Disease/cerebrospinal fluid
Sample handling
Neuroscience (all)
alpha-Synuclein/cerebrospinal fluid
Geriatrics gerontology
business.industry
General Neuroscience
Neurodegenerative Diseases/cerebrospinal fluid
Reproducibility of Results
Biomarker
ELISA
Neurodegenerative Diseases
Parkinson Disease
Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3]
Neurology (clinical)
Developmental Biology
Geriatrics and Gerontology
United States
3. Good health
Europe
chemistry
Homogeneous
Immunology
Female
Human medicine
business
Biomarkers
Zdroj: Neurobiology of aging
Neurobiology of Aging, 36, 2587-96
NEUROBIOL AGING
r-FISABIO: Repositorio Institucional de Producción Científica
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Neurobiology of Aging, 36, 9, pp. 2587-96
NEUROBIOLOGY OF AGING
r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname
Kruse, N, Persson, S, Alcolea, D, Bahl, J M C, Baldeiras, I, Capello, E, Chiasserini, D, Chiavetto, L B, Emersic, A, Engelborghs, S, Eren, E, Fladby, T, Frisoni, G, Garcia-Ayllon, M S, Genc, S, Gkatzima, O, Heegaard, N H H, Janeiro, A M, Kovacech, B, Kuiperij, H B, Leitao, M J, Lleo, A, Martins, M, Matos, M, Mollergard, H M, Nobili, F, Ohrfelt, A, Parnetti, L, de Oliveira, C R, Rot, U, Saez-Valero, J, Struyfs, H, Tanassi, J T, Taylor, P, Tsolaki, M, Vanmechelen, E, Verbeek, M M, Zilka, N, Blennow, K, Zetterberg, H & Mollenhauer, B 2015, ' Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study ', Neurobiology of Aging, vol. 36, no. 9, pp. 2587-2596 . https://doi.org/10.1016/j.neurobiolaging.2015.05.003
r-FISABIO. Repositorio Institucional de Producción Científica
Neurobiology of Aging, Vol. 36, No 9 (2015) pp. 2587-96
r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
ISSN: 0197-4580
DOI: 10.1016/j.neurobiolaging.2015.05.003
Popis: © 2015 Elsevier Inc. All rights reserved.
Decreased levels of alpha-synuclein (aSyn) in cerebrospinal fluid (CSF) in Parkinson's disease and related synucleinopathies have been reported, however, not consistently in all cross-sectional studies. To test the performance of one recently released human-specific enzyme-linked immunosorbent assay (ELISA) for the quantification of aSyn in CSF, we carried out a round robin trial with 18 participating laboratories trained in CSF ELISA analyses within the BIOMARKAPD project in the EU Joint Program - Neurodegenerative Disease Research. CSF samples (homogeneous aliquots from pools) and ELISA kits (one lot) were provided centrally and data reported back to one laboratory for data analysis. Our study showed that although factors such as preanalytical sample handling and lot-to-lot variability were minimized by our study design, we identified high variation in absolute values of CSF aSyn even when the same samples and same lots of assays were applied. We further demonstrate that although absolute concentrations differ between laboratories the quantitative results are comparable. With further standardization this assay may become an attractive tool for comparing aSyn measurements in diverse settings. Recommendations for further validation experiments and improvement of the interlaboratory results obtained are given.
This is an EU Joint Program – Neurodegenerative Disease Research (JPND) project. The project is supported through the following funding organizations under the aegis of JPND–www.jpnd.eu: The Alzheimer Research Foundation (SAO-FRA), Belgium (grant number S#12018); Danish Council for Strategic Research, Denmark (grant number 0603-00511B); Bundesministerium für Bildung und Forschung (BMBF), Germany (grant number OIED1203C); General Secretary of Research and Technology, Greece; Italian Ministry of Health (Ricerca Corrente), Italy; The Fundação para a Ciência e a Tecnologia, Portugal (grant numbers JPND/0004/2011, IMM/BPD/51-2013, IMM/BI/7-2013, JPND/0005/2011); Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Spain (grant numbers PI11/03026, PI11/03035; cofinanced by the Fondo Europeo de Desarrollo Regional); The Ministry of Education, Research and Sport of the Slovak Republic, Slovakia; The Slovenian Research Agency, Slovenia (grant number A3-0001); The Swedish Research Council, Sweden; Ministry of Education for Netherlands: ZonMw (The Netherlands Organisation for Health Research and Development), The Netherlands (grant number 629000002); The Scientific and Technological Research Council of Turkey (grant number 112S335).
Databáze: OpenAIRE
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