Human recombinant Fab fragments with sub-nanomolar affinities for acetylated histones
| Autor: | Betty Diamond, Rena May, Matthew D. Scharff, Czeslawa Kowal, I.N. Batova |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Chromatin Immunoprecipitation
medicine.drug_class Immunology Antibody Affinity Immunoglobulin Variable Region Enzyme-Linked Immunosorbent Assay Biology Cross Reactions Monoclonal antibody Histone H4 Histones Immunoglobulin Fab Fragments Antibody Specificity Cell Line Tumor Histone H2A medicine Immunology and Allergy Histone code Humans Lupus Erythematosus Systemic Autoantibodies Gene Library Histone Acetyltransferases Antibodies Monoclonal Acetylation Molecular biology Recombinant Proteins Histone Biochemistry Polyclonal antibodies Histone methyltransferase biology.protein |
| Zdroj: | Journal of immunological methods. 329(1-2) |
| ISSN: | 0022-1759 |
| Popis: | Acetylation of lysines at different sites in the N-terminus of core histones is a common mode of chromatin modification; different combinations of such modifications are associated with distinct patterns of gene expression, replication and repair. Antibodies are usually used to identify and localize particular histone modifications and to correlate their presence with transcription or other cellular processes. This requires antibodies of sufficient specificity and affinity for each of the many modifications that have now been observed. In most instances, polyclonal antibodies have been used but monoclonal antibodies can also be effective. Here we report that a phage-displayed repertoire of rearranged antibody genes from splenic B cells from a patient with systemic lupus contain Fab fragments that can bind native acetylated lysine 8 histone H4. This finding represents the first selection of human antibodies specific for acetylated histone and suggests that lupus antibodies may contribute to dissection of the histone code. |
| Databáze: | OpenAIRE |
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