The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

Autor: Elisabeth Blesbois, Aurore Thélie, Jean-Claude Poirier, Sophie Réhault-Godbert, Sophie Fouchécourt, Marina Govoroun
Přispěvatelé: Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
acrosin
chicken
male fertility
protease inhibitor
seminal plasma
medicine.medical_treatment
0302 clinical medicine
Biologie de la reproduction
Research Articles
Reproductive Biology
fertilité mâle
030219 obstetrics & reproductive medicine
biology
Reverse Transcriptase Polymerase Chain Reaction
plasma seminal
Acrosin
Spermatozoa
Isoenzymes
Biochemistry
inhibiteur de protéase
Research Article
medicine.drug
Proteases
Trypsin inhibitor
poulet
Semen
Amidohydrolases
03 medical and health sciences
Genetics
medicine
Animals
Amino Acid Sequence
Glycoproteins
Serine protease
Protease
Serine Peptidase Inhibitors
Kazal Type
fertilité animale
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Cell Biology
Sperm
Protease inhibitor (biology)
protéine isoforme
Fertility
030104 developmental biology
biology.protein
Transcriptome
Chickens
Biomarkers
Developmental Biology
Zdroj: Molecular Reproduction and Development 7 (86), 762-775. (2019)
Molecular Reproduction and Development
Molecular Reproduction and Development, Wiley, 2019, 86 (7), pp.762-775. ⟨10.1002/mrd.23153⟩
ISSN: 1098-2795
1040-452X
DOI: 10.1002/mrd.23153
Popis: International audience; The seminal plasma is a very complex fluid, which surrounds sperm in semen. It contains numerous proteins including proteases and protease inhibitors that regulate proteolytic processes associated with protein activation and degradation. We previously identified a seminal protein, chicken liver trypsin inhibitor 1 (ClTI‐1) over expressed in semen of roosters with high fertility, suggesting a role in male fertility. In the present study, we showed that ClTI‐1 gene is actually SPINK2. Using normal healthy adult roosters, we showed that SPINK2 amount in seminal plasma was positively correlated with male fertility in chicken lines with highly contrasted genetic backgrounds (broiler and layer lines). Using affinity chromatography combined to mass spectrometry analysis and kinetic assays, we demonstrated for the first time that two chicken acrosin isoforms (acrosin and acrosin‐like proteins) are the physiological serine protease targets of SPINK2 inhibitor. SPINK2 transcript was overexpressed all along the male tract, and the protein was present in the lumen as expected for secreted proteins. Altogether, these data emphasize the role of seminal SPINK2 Kazal‐type inhibitor as an important actor of fertility in birds through its inhibitory action on acrosin isoforms proteins.
Databáze: OpenAIRE
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