RNA-seq analysis of lncRNA-controlled developmental gene expression during puberty in goat & rat
| Autor: | Jing Ye, Fugui Fang, Ya Liu, Xiaoxiao Gao, Xiaorong Zhang, Kaifa Zhang, Lei Luo, Zubing Cao, Chen Yang, Jie Zhou, Yunhai Zhang, Weiping Huang, Yinghui Ling, Xiumei Li, Jianping Ding |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Hypothalamus 030209 endocrinology & metabolism RNA-Seq Pheromone receptor activity Biology 03 medical and health sciences Exon 0302 clinical medicine lncRNA Gene expression Genetics Animals XLOC_446331 Genes Developmental RNA Messenger Sexual Maturation KEGG Gene Genetics (clinical) Messenger RNA Sequence Analysis RNA Gene Expression Profiling Goats Puberty Gene Expression Regulation Developmental Rats Open reading frame lcsh:Genetics 030104 developmental biology Gene Ontology Female RNA Long Noncoding Research Article Signal Transduction |
| Zdroj: | BMC Genetics BMC Genetics, Vol 19, Iss 1, Pp 1-9 (2018) |
| ISSN: | 1471-2156 |
| Popis: | Background Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability. However, little is known about the function of lncRNAs (long noncoding RNAs) in puberty. Therefore, RNA-seq analysis were performed between goats and rats to clarify the roles of lncRNAs and mRNAs in the onset of puberty. Results In the present study, the length of lncRNAs, the length of the open reading frame and the exon count were compared between the two species. Furthermore, functional annotation analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analysis of lncRNAs target genes and differentially expressed mRNA demonstrated the significantly enriched terms, such as AMPK signaling pathway, oxytocin signaling pathway, insulin secretion as well as pheromone receptor activity, and some other signaling pathways which were involved in the regulation of female puberty. Moreover, our results of siRNA interference in vitro showed the candidate lncRNA XLOC_446331 may play a crucial role in regulating female puberty. Conclusion In conclusion, the RNA-seq analysis between goat and rat provide novel candidate regulators for genetic and molecular studies on female puberty. Electronic supplementary material The online version of this article (10.1186/s12863-018-0608-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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