Optimal Ultrasound Exposure Conditions for Maximizing C2C12 Muscle Cell Proliferation and Differentiation
| Autor: | A. Cafarelli, Lorenzo Capineri, Arianna Menciassi, Alice Rita Salgarella, Paolo Dario, Leonardo Ricotti |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Intensity Materials science Acoustics and Ultrasonics Myoblasts Skeletal Ultrasonic Therapy Cellular differentiation Low-intensity pulsed ultrasound Proliferation 0206 medical engineering Biophysics Bio-effects Stimulation 02 engineering and technology Radiation Dosage Ultrasound stimulation Cell Line Myoblasts Mice 03 medical and health sciences Muscle regeneration Nuclear Medicine and Imaging medicine Animals Myocyte Radiology Nuclear Medicine and imaging Cell Proliferation Radiological and Ultrasound Technology Cell growth business.industry Muscle cell proliferation Ultrasound Cell Differentiation Dose-Response Relationship Radiation Frequency Radiation Exposure 020601 biomedical engineering C2C12 Differentiation Radiology Nuclear Medicine and Imaging 030104 developmental biology Ultrasonic Waves Radiology business Biomedical engineering |
| Zdroj: | Ultrasound in Medicine & Biology. 43:1452-1465 |
| ISSN: | 0301-5629 |
| Popis: | Described here is an in vitro systematic investigation of the effects on C2C12 myoblasts of exposure to finely controlled and repeatable low-intensity pulsed ultrasound of different frequencies (500 kHz, 1 MHz, 3 MHz and 5 MHz) and different intensities (250, 500 and 1000 mW/cm2). An in-house stimulation system and an ultrasound-transparent cell culture well minimized reflections and attenuations, allowing precise control of ultrasound delivery. Results indicated that a 3 MHz stimulation at 1 W/cm2 intensity maximized cell proliferation in comparison with the other exposure conditions and untreated controls. In contrast, cell differentiation and the consequent formation of multinucleated myotubes were maximized by 1 MHz stimulation at 500 mW/cm2 intensity. The highly controlled exposure conditions employed allowed precise correlation of the ultrasound delivery to the bio-effects produced, thus overcoming the inconsistency of some results available in the literature and contributing to the potential of ultrasound treatment for muscle therapy and regeneration. |
| Databáze: | OpenAIRE |
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