Prospecting Anticancer Compounds in Actinomycetes Recovered from the Sediments of Saint Peter and Saint Paul's Archipelago, Brazil
| Autor: | Letícia V. Costa-Lotufo, Elthon G. Ferreira, Otília Deusdênia L. Pessoa, Edilberto R. Silveira, Larissa L. F. Colares, Alison B. da Silva, Karine Pires, Paula C. Jimenez, Tito Monteiro da Cruz Lotufo, Maria Conceição M. Torres |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Geologic Sediments Molecular Conformation Bioengineering Rifamycins Fractionation 01 natural sciences Biochemistry Streptomyces FARMACOLOGIA 03 medical and health sciences Structure-Activity Relationship Botany Ic50 values Prospecting Humans Molecular Biology Cell Proliferation geography geography.geographical_feature_category biology Dose-Response Relationship Drug 010405 organic chemistry Chemistry Sediment General Chemistry General Medicine biology.organism_classification HCT116 Cells Antineoplastic Agents Phytogenic 0104 chemical sciences Salinisporamycin SEDIMENTOLOGIA MARINHA Actinobacteria 030104 developmental biology Archipelago Molecular Medicine Drug Screening Assays Antitumor Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1612-1880 |
| Popis: | Saint Peter and Saint Paul's Archipelago is a collection of 15 islets and rocks remotely located in the equatorial Atlantic Ocean. In this particular site, the present project intended to assess the biodiversity and biotechnological potential of bacteria from the actinomycete group. This study presents the first results of this assessment. From 21 sediment samples, 268 strains were isolated and codified as BRA followed by three numbers. Of those, 94 strains were grown in liquid media and submitted to chemical extractions with AcOEt (A), BuOH (B), and MeOH (M). A total of 224 extracts were screened for their cytotoxic activity and 41 were significantly active against HCT-116 cancer cells. The obtained IC50 values ranged from 0.04 to 31.55 μg/ml. The HR-LC/MS dereplication analysis of the active extracts showed the occurrence of several known anticancer compounds. Individual compounds, identified using HR-MS combined with analysis of the AntiMarin database, included saliniketals A and B, piericidins A and C and glucopiericidin A, staurosporine, N-methylstaurosporine, hydroxydimethyl-staurosporine and N-carbamoylstaurosporine, salinisporamycin A, and rifamycins S and B. BRA-199, identified as Streptomyces sp., was submitted to bioassay-guided fractionation, leading to isolation of the bioactive piericidins A and C, glucopiericidin, and three known diketopiperazines, cyclo(l-Phe-trans-4-OH-l-Pro), cyclo(l-Phe-l-Pro), and cyclo(l-Trp-l-Pro). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text