Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry
| Autor: | Jessica R Hughes, Todd W. Mitchell, Roger J.W. Truscott, Stephen J. Blanksby, Alan T. Maccarone, Jo Ann Seng, Shane R. Ellis |
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| Rok vydání: | 2013 |
| Předmět: |
Electrospray
Spectrometry Mass Electrospray Ionization Sphingolipids Chromatography music.instrument Chemistry Lens (geology) Cell Biology Lipidome Mass spectrometry Sphingolipid Thin-layer chromatography Lactosylceramide Young Adult Cholesterol Biochemistry Lipidomics Lens Crystalline Humans lipids (amino acids peptides and proteins) Chromatography Thin Layer music Molecular Biology |
| Zdroj: | Biochimica et biophysica acta. 1841(9) |
| ISSN: | 0006-3002 |
| Popis: | The lipidome of the human lens is unique in that cholesterol and dihydrosphingomyelin are the dominant classes. Moreover, the lens lipidome is not static with dramatic changes in several sphingolipid classes associated with both aging and cataract. Accordingly, there is a clear need to expand knowledge of the molecular species that constitute the human lens sphingolipidome. In this study, human lens lipids have been extracted and separated by thin-layer chromatography (TLC). Direct analysis of the TLC plates by desorption electrospray ionisation-mass spectrometry (DESI-MS) allowed the detection over 30 species from 11 classes of sphingolipids. Significantly, novel classes of lens lipids including sulfatides, dihydrosulfatides, lactosylceramide sulfates and dihydrolactosylceramide sulfates were identified. |
| Databáze: | OpenAIRE |
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