Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
| Autor: | Letícia de Almeida Brondani, Tor Gunnar Hugo Onsten, Francyne Kubaski, Leo Sekine, Natália Emerim Lemos, Luciana Scotti, Mariela Granero Farias, Sandrine Comparsi Wagner |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Oncology
medicine.medical_specialty CD34 030204 cardiovascular system & hematology Single Center Flow cytometry 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Immunology and Allergy Progenitor cell Transplantation medicine.diagnostic_test lcsh:RC633-647.5 business.industry lcsh:Diseases of the blood and blood-forming organs Hematology Leukapheresis Apheresis CD34+ progenitor cells Stem cell business 030215 immunology |
| Zdroj: | Hematology, Transfusion and Cell Therapy, Vol 40, Iss 3, Pp 213-218 (2018) Hematology, Transfusion and Cell Therapy, Volume: 40, Issue: 3, Pages: 213-218, Published: SEP 2018 |
| ISSN: | 2531-1379 |
| Popis: | Background: Due to laboratory logistic issues, our center has traditionally scheduled peripheral blood stem cell harvests based on timing from the start of mobilization. This has proved to be useful in some cases, but also resulted in many fruitless harvests due to poor mobilization. In order to improve the efficiency of collections and compare the effectiveness of peripheral blood CD34+ cells as a predictor with data from other reports, this study analyzed the implementation of this routine. Methods: Peripheral blood and leukapheresis samples were quantified by flow cytometry and the association between these parameters was assessed. Results: Sixty-six consecutive leukapheresis samples were collected from 34 patients after the collection of peripheral blood samples for CD34+ quantification. A moderate positive correlation was observed between peripheral blood CD34+ cell count and total CD34+ cell count/kg (r = 0.596; p-value |
| Databáze: | OpenAIRE |
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