Altered Cerebrospinal Fluid Concentrations of Hydrophobic and Hydrophilic Compounds in Early Stages of Multiple Sclerosis—Metabolic Profile Analyses
| Autor: | Aleksandra Podlecka-Piętowska, Beata Toczylowska, Małgorzata Chalimoniuk, Elzbieta Zieminska, Beata Zakrzewska-Pniewska, Monika Nojszewska, A. Kacka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Multiple Sclerosis Pharmacology Article Choline Acetone 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound NMR spectroscopy 0302 clinical medicine Cerebrospinal fluid Metabolomics medicine Humans Urea Amino Acids Inflammation chemistry.chemical_classification Multiple sclerosis Fatty acid General Medicine Middle Aged medicine.disease Lipids Metabolic pathway 030104 developmental biology chemistry Case-Control Studies Creatinine Metabolome Female Leucine Isoleucine Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Journal of Molecular Neuroscience |
| ISSN: | 1559-1166 0895-8696 |
| Popis: | The lack of a single predictive or diagnostic test in multiple sclerosis (MS) remains a major obstacle in the patient’s care. The aim of this study was to investigate metabolic profiles, especially lipids in cerebrospinal fluid (CSF) using 1H-NMR spectroscopy and metabolomics analysis to discriminate MS patient group from the control ones. In this study, 19 MS patients and 19 controls, without neurological problems, patients were enrolled. To obtain the CSF metabolic profiles, NMR spectroscopy was used. Hydrophilic and hydrophobic compounds were analyzed using univariate and multivariate supervised analysis orthogonal partial least square discriminant analysis (OPLS-DA). Targeted OPLS-DA analysis of 32 hydrophilic and 17 hydrophobic compounds obtained 9 hydrophilic metabolites and 8 lipid functional groups which had the highest contribution to patient’s group separation. Lower concentrations of CSF hydrophilic and hydrophobic compounds were observed in MS patients as compared to control group. Acetone, choline, urea, 1,3-dimethylurate, creatinine, isoleucine, myo-inositol, leucine, and 3-OH butyrate; saturated and monounsaturated acyl groups of ω–9, ω–7, ω–6, ω–3, and fatty acid, triglycerides, 1,3-DG, 1-MG, and unassigned component signal at 3.33 ppm were the most important signal compounds in group separation. Analysis of metabolic profile of raw CSF and their lipid extract shows decreased levels of many compounds and led to the conclusion that MS patients could have a disturbance in many metabolic pathways perhaps leading to the decreased level of acetyl-CoA and/or inflammation. CSF metabolic profile analyses could be used as a fingerprint for early MS diagnosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink