THE CORRELATION BETWEEN TERPENOID OF SARANG SEMUT (Myrmecodia pendans) WITH mRNA FRUCTOSYLTRANFERASE EXPRESSION OF Streptococcus mutans BIOFILM ATCC 25175
| Autor: | Darul Rabil, Eka Chemiawan, Mieke Hemiawati Satari, Meirina Gartika |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Messenger RNA
biology Natural materials ekspresi mrna ftf Mrna expression Significant difference fungi Biofilm RK1-715 Myrmecodia biology.organism_classification Streptococcus mutans Terpenoid Microbiology body regions biofilm streptoccus mutans terpenoid dari sarang semut stomatognathic system Dentistry |
| Zdroj: | ODONTO: Dental Journal, Vol 8, Iss 1, Pp 86-93 (2021) |
| ISSN: | 2460-4119 2354-5992 |
| Popis: | Background: Terpenoid isolate sarang semut (Myrmecodia pendans) is a natural material that has antibacterial power through the destruction of the cell membrane of Streptoccus mutans (S. mutans). Fructosiltransferase (ftf) is one of the extrapolisaccharides produced by S. mutans. The formation of Ftf is governed by the ftf gene. The aim of research was to know the correlation between terpenoid of Sarang semut administration with ftf mRNA expression of S. mutans biofilm ATCC 25175. Method: The study used mixed methods, namely descriptive explorative method to look at the isolation of ftf gene and ftf mRNA expression. This study began with making 1 day biofilm , then given terpenoid isolates of sarang semut and chlorhexidine. Next, isolation of ftf genes and ftf mRNA expression was carried out, then the differences in S. mutans ATCC 25175 mRNA ftf expression in sarang semut and chlorhexidine were given for 1 and 30 minutes using Real-Time PCR. Continued by looking at the relationship of the duration of administration of sarang semut terpenoids to the expression of S. mutans ATCC 25175 mRNA ftf biofilm. Statistical analysis used the t test with p-value |
| Databáze: | OpenAIRE |
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