Reduction of Streptococcus mutans Adherence and Dental Biofilm Formation by Surface Treatment with Phosphorylated Polyethylene Glycol
| Autor: | Yoshinori Takaesu, Takashi Matsukubo, Hisataka Kobayashi, Takashi Inoue, Tadashi Hase, Junji Nakamura, Akira Shimotoyodome, Takashi Koudate, Ichiro Tokimitsu |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Saliva Surface Properties Dental Plaque Histatins Polyethylene glycol Pyrophosphate Bacterial Adhesion Polyethylene Glycols Microbiology Streptococcus mutans chemistry.chemical_compound stomatognathic system PEG ratio Animals Humans Parotid Gland Experimental Therapeutics Pharmacology (medical) Amino Acids Phosphorylation Salivary Proteins and Peptides Pharmacology Enamel paint biology Biofilm Adhesion biology.organism_classification Rats stomatognathic diseases Durapatite Infectious Diseases chemistry Biochemistry Biofilms Drug Design visual_art visual_art.visual_art_medium Salivation Tooth |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 51:3634-3641 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00380-07 |
| Popis: | Initial attachment of the cariogenic Streptococcus mutans onto dental enamel is largely promoted by the adsorption of specific salivary proteins on enamel surface. Some phosphorylated salivary proteins were found to reduce S. mutans adhesion by competitively inhibiting the adsorption of S. mutans -binding salivary glycoproteins to hydroxyapatite (HA). The aim of this study was to develop antiadherence compounds for preventing dental biofilm development. We synthesized phosphorylated polyethylene glycol (PEG) derivatives and examined the possibility of surface pretreatment with them for preventing S. mutans adhesion in vitro and dental biofilm formation in vivo. Pretreatment of the HA surface with methacryloyloxydecyl phosphate (MDP)-PEG prior to saliva incubation hydrophilized the surface and thereby reduced salivary protein adsorption and saliva-promoted bacterial attachment to HA. However, when MDP-PEG was added to the saliva-pretreated HA (S-HA) surface, its inhibitory effect on bacterial binding was completely diminished. S. mutans adhesion onto S-HA was successfully reduced by treatment of the surface with pyrophosphate (PP), which desorbs salivary components from S-HA. Treatment of S-HA surfaces with MDP-PEG plus PP completely inhibited saliva-promoted S. mutans adhesion even when followed by additional saliva treatment. Finally, mouthwash with MDP-PEG plus PP prevented de novo biofilm development after thorough teeth cleaning in humans compared to either water or PP alone. We conclude that MDP-PEG plus PP has the potential for use as an antiadherence agent that prevents dental biofilm development. |
| Databáze: | OpenAIRE |
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