A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity
| Autor: | Lihua Lu, Bingyong He, Sheng Lin, Dik-Lung Ma, Chung-Hang Leung |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Nuclease Aqueous solution biology Biomedical Engineering chemistry.chemical_element General Chemistry General Medicine Combinatorial chemistry Highly sensitive chemistry.chemical_compound Crystallography Enzyme chemistry Mung Bean Nuclease biology.protein General Materials Science Iridium Luminescence DNA |
| Zdroj: | Journal of Materials Chemistry B. 3:348-352 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c4tb01569j |
| Popis: | A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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