Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Autor: Gönül Tezcan Keleş, Seçil Erden Tayhan, Saime İsmet Deliloğlu Gürhan, İsmet Topçu, Erol Mir
Přispěvatelé: Department of Bioengineering, Gaziosmanpaşa University, Faculty of Natural Sciences and Engineering, Tokat, Turkey, Department of Anesthesiology and Reanimation, Celal Bayar University School of Medicine, Manisa, Turkey, Department of Pediatric Surgery, Celal Bayar University School of Medicine, Manisa, Turkey, Department of Bioengineering, Ege University, Faculty of Engineering, İzmir, Turkey, Ege Üniversitesi, Gaziosmanpaşa Üniversitesi, 0-Belirlenecek
Rok vydání: 2017
Předmět:
Zdroj: Türk Üroloji Dergisi/Turkish Journal of Urology. 43:345-349
ISSN: 2149-3057
2149-3235
DOI: 10.5152/tud.2017.93797
Popis: WOS: 000410005000021
PubMed ID: 28861309
Objective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. Material and methods: After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies. Results: When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine-derived cell population. Conclusion: According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method.
TUBITAK-BIDEB National Scholarship Programme [2211]
Secil Erden Tayhan, one of the researchers of this study, was finantially supported by TUBITAK-BIDEB 2211 National Scholarship Programme for PhD Students.
Databáze: OpenAIRE
Externí odkaz: