P38/NF-κB/snail pathway is involved in caffeic acid-induced inhibition of cancer stem cells-like properties and migratory capacity in malignant human keratinocyte
| Autor: | Wenqin Yin, Lei Li, Kebo Wang, Yuan Li, Yu Wang, Ye Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Keratinocytes
lcsh:Medicine Snail p38 Mitogen-Activated Protein Kinases Metastasis Molecular cell biology Signal Initiation Cell Movement Basic Cancer Research Signaling in Cellular Processes Phosphorylation lcsh:Science Skin Tumors Cellular Stress Responses Multidisciplinary biology Mechanisms of Signal Transduction Statistics NF-kappa B Nuclear Signaling Blot Cell Transformation Neoplastic Phenotype Oncology Neoplastic Stem Cells Medicine Cell Movement Signaling Research Article Signal Transduction DNA transcription Phosphoinositide Signal Transduction Biostatistics Signaling Pathways Cell Line Caffeic Acids Cancer stem cell biology.animal mental disorders medicine Anticarcinogenic Agents Humans Neoplastic transformation cardiovascular diseases Biology lcsh:R Cancer Cancers and Neoplasms nutritional and metabolic diseases medicine.disease Molecular biology HaCaT Cell culture Cell Transdifferentiation Cancer research lcsh:Q Gene expression Transcriptional Signaling Snail Family Transcription Factors Mathematics Transcription Factors |
| Zdroj: | PLoS ONE, Vol 8, Iss 3, p e58915 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background Skin cancer is the most common cancer throughout the world. The epithelial-mesenchymal transition (EMT) and the acquisition of cancer stem cells (CSCs)-like properties emerge as critical steps in the metastasis of human skin cancers. Caffeic acid (CaA) exerts anticarcinogenic effects. However, the effects of CaA on the migratory capability and on the CSCs-like properties of skin cancer cells, and the molecular mechanisms underlying it are not fully understood. Methods Malignant HaCaT cells were treated by CaA. Transwell assay was performed to determine that CaA attenuated the migratory capability; Spheroid formation assay was performed to confirm that CaA decreased the CSCs-like phenotype; Treated malignant HaCaT cells were molecularly characterized by RT-PCR, Western blots, Southwestern blot, and immunoprecipitation. Results In CaA-treated malignant human keratinocyte (malignant HaCaT cells), inhibition of the migratory capability and CSCs-like phenotype were observed. CaA up-regulated the phosphorylation of p38, and down-regulated the activation of nuclear factor κB (NF-κB)/snail signal pathway. Indeed, p38 decreased the DNA-binding activity of NF-κB to the promoter of snail gene, which resulted in the transcriptional inactivation of snail. Blockage of p38 attenuated the CaA-induced inhibition of migratory capability and CSCs-like phenotype in malignant HaCaT cells. Conclusions CaA attenuates the migratory capability and CSCs-like Properties of malignant human keratinocyte, in which, p38-mediated down-regulation of NF-κB/snail signal pathway is involved. |
| Databáze: | OpenAIRE |
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