Macroautophagy Proteins Assist Epstein Barr Virus Production and Get Incorporated Into the Virus Particles
| Autor: | Kerstin Thriene, Christian Münz, Urs Ziegler, Heike Nowag, Joern Dengjel, Bruno Guhl, Susana Romao |
|---|---|
| Přispěvatelé: | University of Zurich, Münz, Christian |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
viruses
Atg8/LC3 lcsh:Medicine 10263 Institute of Experimental Immunology medicine.disease_cause BALF1 BamH1 A fragment leftward reading frame 1 Atg autophagy related gene 0302 clinical medicine 0303 health sciences education.field_of_study B cell lcsh:R5-920 BHRF1 BamH1 H fragment rightward reading frame 1 BMRF1 BamH1 M fragment rightward reading frame 1 General Medicine 3. Good health Lytic cycle 030220 oncology & carcinogenesis BRLF1 BamH1 R fragment leftward reading frame 1 Original Article 10024 Center for Microscopy and Image Analysis Epstein–Barr virus nuclear antigen 1 lcsh:Medicine (General) LMP1 latent membrane protein 1 ATG8 Population Lytic EBV replication Atg16 EBNA1 Epstein Barr virus nuclear antigen 1 610 Medicine & health Biology BZLF1 General Biochemistry Genetics and Molecular Biology Virus 03 medical and health sciences EBV Epstein Barr virus 1300 General Biochemistry Genetics and Molecular Biology Atg12 medicine education BZLF1 BamH1 Z fragment leftward reading frame 1 030304 developmental biology BNRF1 BamH1 N fragment rightward reading frame 1 vFLIP viral FLICE-like inhibitor protein lcsh:R Epstein–Barr virus Virology Epithelial cell Cytosol BALF4 BamH1 A fragment leftward reading frame 4 570 Life sciences biology |
| Zdroj: | EBioMedicine, Vol 1, Iss 2, Pp 116-125 (2014) EBioMedicine |
| ISSN: | 2352-3964 |
| Popis: | Epstein Barr virus (EBV) persists as a latent herpes virus infection in the majority of the adult human population. The virus can reactivate from this latent infection into lytic replication for virus particle production. Here, we report that autophagic membranes, which engulf cytoplasmic constituents during macroautophagy and transport them to lysosomal degradation, are stabilized by lytic EBV replication in infected epithelial and B cells. Inhibition of autophagic membrane formation compromises infectious particle production and leads to the accumulation of viral DNA in the cytosol. Vice versa, pharmacological stimulation of autophagic membrane formation enhances infectious virus production. Atg8/LC3, an essential macroautophagy protein and substrate anchor on autophagic membranes, was found in virus preparations, suggesting that EBV recruits Atg8/LC3 coupled membranes to its envelope in the cytosol. Our data indicate that EBV subverts macroautophagy and uses autophagic membranes for efficient envelope acquisition during lytic infection. Highlights • Macroautophagic membranes are stabilized during lytic EBV replication. • Inhibition of macroautophagic membrane formation reduces EBV production. • Stimulation of macroautophagic membrane formation boosts EBV production. • Without macroautophagic membranes EBV DNA accumulates in the cytosol. • Macroautophagic membranes get incorporated into EBV particles. |
| Databáze: | OpenAIRE |
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