Lymphatic and blood microvasculature organisation in pulmonary sarcoid granulomas
Autor: | Dominique Valeyre, Laurentiu Mogoanta, Marianne Kambouchner, Jean-François Bernaudin, Uhl Jf, Daniel Pirici |
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Rok vydání: | 2010 |
Předmět: |
Pulmonary and Respiratory Medicine
Adult Male Pathology medicine.medical_specialty Biopsy Context (language use) Antigens CD34 Antibodies Monoclonal Murine-Derived stomatognathic system Sarcoidosis Pulmonary Fibrosis hemic and lymphatic diseases Image Processing Computer-Assisted Medicine Humans Lymphatic Vessels Retrospective Studies Lung Granuloma business.industry Microcirculation Fibrous ring Middle Aged medicine.disease Platelet Endothelial Cell Adhesion Molecule-1 Lymphatic system medicine.anatomical_structure Female business |
Zdroj: | The European respiratory journal. 37(4) |
ISSN: | 1399-3003 |
Popis: | Pulmonary sarcoid granulomas are characterised by their elective distribution along collecting lymphatics. However, relationships between granulomas and intralobular lymphatics or blood microvascularisation have not been investigated. Therefore, we undertook a specific analysis of blood capillaries and lymphatics supplying sarcoid granulomas to identify additional clues to understanding the pathophysiogenesis of these lesions. Six pulmonary samples were immunolabelled with D2-40, anti-CD34 and anti-CD31 antibodies, paying particular attention to the relationships between lymphatics and granulomas, and the pattern of blood microvessels supplying sarcoid lesions. A morphometric study of granulomas included their distance to lymphatics and a three-dimensional reconstruction of a granuloma in its lymphatic context. Intralobular granulomas were closely associated with lymphatics; apart from a few granulomas, blood capillaries stopped at the outer border of the fibrous ring surrounding granulomas, and perigranuloma capillaries were particularly scarce. Our observations of the lymphatic and blood microvascular environment of intralobular pulmonary sarcoid granulomas provide evidence for the critical role of lymphatics in the emergence of these lesions. Moreover, pulmonary sarcoid lesions could be considered avascular structures, thereby providing new insights into the understanding of the granuloma physiology and the distribution of blood-borne therapeutic agents. |
Databáze: | OpenAIRE |
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