Multifocal structure of the T cell - dendritic cell synapse
| Autor: | Alain Schmitt, Clotilde Randriamampita, Cédric Brossard, Alain Trautmann, Graça Raposo, Maryse Romao, Vincent Feuillet |
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| Přispěvatelé: | [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
CD3 Complex
T cell T-Lymphocytes Immunology Fluorescent Antibody Technique [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Immunofluorescence law.invention Synapse 03 medical and health sciences Mice 0302 clinical medicine law medicine Immunology and Allergy Animals Cytoskeleton Antigen-presenting cell ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences medicine.diagnostic_test Dendritic cell Dendritic Cells Intercellular Adhesion Molecule-1 Immunological Synapses Lymphocyte Function-Associated Antigen-1 Cell biology Mice Inbred C57BL Microscopy Electron medicine.anatomical_structure Synapses [SDV.IMM]Life Sciences [q-bio]/Immunology Electron microscope 030215 immunology |
| Zdroj: | European Journal of Immunology European Journal of Immunology, Wiley-VCH Verlag, 2005, 35 (6), pp.1741-1753. ⟨10.1002/eji.200425857⟩ |
| ISSN: | 0014-2980 1521-4141 |
| DOI: | 10.1002/eji.200425857⟩ |
| Popis: | The structure of immunological synapses formed between murine naive T cells and mature dendritic cells has been subjected to a quantitative analysis. Immunofluorescence images of synapses formed in the absence of antigen show a diffuse synaptic accumulation of CD3 and LFA-1. In electron microscopy, these antigen-free synapses present a number of tight appositions (cleft size approximately 15 nm), all along the synapse. These tight appositions cover a significantly larger surface fraction of antigen-dependent synapses. In immunofluorescence, antigen-dependent synapses show multiple patches of CD3 and LFA-1 with a variable overlap. A similar distribution is observed for PKCtheta and talin. A concentric organization characteristic of prototypical synapses is rarely observed, even when dendritic cells are paralyzed by cytoskeletal poisons. In T-DC synapses, the interaction surface is composed of several tens of submicronic contact spots, with no large-scale segregation of CD3 and LFA-1. As a comparison, in T-B synapses, a central cluster of CD3 is frequently observed by immunofluorescence, and electron microscopy reveals a central tight apposition. Our data show that it is inappropriate to consider the concentric structure as a "mature synapse" and multifocal structures as immature. |
| Databáze: | OpenAIRE |
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