Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

Autor:	Jerzy Wiater, Malgorzata Duda, Gabriela Gorczyca, Zbigniew Tabarowski, Kamil Wartalski
Rok vydání:	2021
Předmět:	0301 basic medicine Histology Swine Cellular differentiation medicine.medical_treatment Endothelial cells Basic fibroblast growth factor Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Epidermal growth factor medicine Cell differentiation Animals CD90 Molecular Biology Original Paper Pig Putative stem cells Growth factor Stem Cells Mesenchymal stem cell Ovary Cell Biology Cell biology Vascular endothelial growth factor Medical Laboratory Technology 030104 developmental biology chemistry 030220 oncology & carcinogenesis Pituitary Gland Female Stem cell
Zdroj:	Histochemistry and Cell Biology
ISSN:	1432-119X
Popis:	Endothelial cells (ECs), the primary component of the vasculature, play a crucial role in neovascularization. However, the number of endogenous ECs is inadequate for both experimental purposes and clinical applications. Porcine ovarian putative stem cells (poPSCs), although not pluripotent, are characterized by great plasticity. Therefore, this study aimed to investigate whether poPSCs have the potential to differentiate into cells of endothelial lineage. poPSCs were immunomagnetically isolated from postnatal pig ovaries based on the presence of SSEA-4 protein. Expression of mesenchymal stem cells (MSCs) markers after pre-culture, both at the level of mRNA: ITGB1, THY, and ENG and corresponding protein: CD29, CD90, and CD105 were significantly higher compared to the control ovarian cortex cells. To differentiate poPSCs into ECs, inducing medium containing vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), ascorbic acid, and heparin was applied. After 14 days, poPSC differentiation into ECs was confirmed by immunofluorescence staining for vascular endothelial cadherin (VECad) and vascular endothelial growth factor receptor-2 (VEGFR-2). Semi-quantitative WB analysis of these proteins confirmed their high abundance. Additionally, qRT-PCR showed that mRNA expression of corresponding marker genes: CDH5, KDR was significantly higher compared with undifferentiated poPSCs. Finally, EC functional status was confirmed by the migration test that revealed that they were capable of positive chemotaxis, while tube formation assay demonstrated their ability to develop capillary networks. In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b145ffcd14c263975ae9828d3c1eafd https://pubmed.ncbi.nlm.nih.gov/34269874 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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